Antigenic composition of single nano-sized extracellular blood vesicles

Anush Arakelyan, Oxana Ivanova, Elena Vasilieva, Jean-Charles B. Grivel, Leonid Margolis

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) are important in normal physiology and are altered in various pathologies. EVs produced by different cells are antigenically different. Since the majority of EVs are too small for routine flow cytometry, EV composition is studied predominantly in bulk, thus not addressing their antigenic heterogeneity. Here, we describe a nanoparticle-based technique for analyzing antigens on single nano-sized EVs. The technique consists of immuno-capturing of EVs with 15-nm magnetic nanoparticles, staining captured EVs with antibodies against their antigens, and separating them from unbound EVs and free antibodies in a magnetic field, followed by flow analysis. This technique allows us to characterize EVs populations according to their antigenic distribution, including minor EV fractions. We demonstrated that the individual blood EVs carry different sets of antigens, none being ubiquitous, and quantified their distribution. The physiological significance of antigenically different EVs and their correlation with different pathologies can now be directly addressed.

Original languageEnglish
Pages (from-to)489-498
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume11
Issue number3
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

Antigens
Blood
Pathology
Antibodies
Chemical analysis
Nanoparticles
Flow cytometry
Physiology
Magnetic fields
Extracellular Vesicles
Magnetic Fields
Flow Cytometry
Staining and Labeling

Keywords

  • Antigenic make-up
  • Exosomes
  • Extracellular vesicles
  • Flow cytometry
  • Microvesicles

ASJC Scopus subject areas

  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Medicine (miscellaneous)
  • Pharmaceutical Science
  • Medicine(all)

Cite this

Antigenic composition of single nano-sized extracellular blood vesicles. / Arakelyan, Anush; Ivanova, Oxana; Vasilieva, Elena; Grivel, Jean-Charles B.; Margolis, Leonid.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 11, No. 3, 2015, p. 489-498.

Research output: Contribution to journalArticle

Arakelyan, Anush ; Ivanova, Oxana ; Vasilieva, Elena ; Grivel, Jean-Charles B. ; Margolis, Leonid. / Antigenic composition of single nano-sized extracellular blood vesicles. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2015 ; Vol. 11, No. 3. pp. 489-498.
@article{ae5dd371e3754f54b0dc91aff306df75,
title = "Antigenic composition of single nano-sized extracellular blood vesicles",
abstract = "Extracellular vesicles (EVs) are important in normal physiology and are altered in various pathologies. EVs produced by different cells are antigenically different. Since the majority of EVs are too small for routine flow cytometry, EV composition is studied predominantly in bulk, thus not addressing their antigenic heterogeneity. Here, we describe a nanoparticle-based technique for analyzing antigens on single nano-sized EVs. The technique consists of immuno-capturing of EVs with 15-nm magnetic nanoparticles, staining captured EVs with antibodies against their antigens, and separating them from unbound EVs and free antibodies in a magnetic field, followed by flow analysis. This technique allows us to characterize EVs populations according to their antigenic distribution, including minor EV fractions. We demonstrated that the individual blood EVs carry different sets of antigens, none being ubiquitous, and quantified their distribution. The physiological significance of antigenically different EVs and their correlation with different pathologies can now be directly addressed.",
keywords = "Antigenic make-up, Exosomes, Extracellular vesicles, Flow cytometry, Microvesicles",
author = "Anush Arakelyan and Oxana Ivanova and Elena Vasilieva and Grivel, {Jean-Charles B.} and Leonid Margolis",
year = "2015",
doi = "10.1016/j.nano.2014.09.020",
language = "English",
volume = "11",
pages = "489--498",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Antigenic composition of single nano-sized extracellular blood vesicles

AU - Arakelyan, Anush

AU - Ivanova, Oxana

AU - Vasilieva, Elena

AU - Grivel, Jean-Charles B.

AU - Margolis, Leonid

PY - 2015

Y1 - 2015

N2 - Extracellular vesicles (EVs) are important in normal physiology and are altered in various pathologies. EVs produced by different cells are antigenically different. Since the majority of EVs are too small for routine flow cytometry, EV composition is studied predominantly in bulk, thus not addressing their antigenic heterogeneity. Here, we describe a nanoparticle-based technique for analyzing antigens on single nano-sized EVs. The technique consists of immuno-capturing of EVs with 15-nm magnetic nanoparticles, staining captured EVs with antibodies against their antigens, and separating them from unbound EVs and free antibodies in a magnetic field, followed by flow analysis. This technique allows us to characterize EVs populations according to their antigenic distribution, including minor EV fractions. We demonstrated that the individual blood EVs carry different sets of antigens, none being ubiquitous, and quantified their distribution. The physiological significance of antigenically different EVs and their correlation with different pathologies can now be directly addressed.

AB - Extracellular vesicles (EVs) are important in normal physiology and are altered in various pathologies. EVs produced by different cells are antigenically different. Since the majority of EVs are too small for routine flow cytometry, EV composition is studied predominantly in bulk, thus not addressing their antigenic heterogeneity. Here, we describe a nanoparticle-based technique for analyzing antigens on single nano-sized EVs. The technique consists of immuno-capturing of EVs with 15-nm magnetic nanoparticles, staining captured EVs with antibodies against their antigens, and separating them from unbound EVs and free antibodies in a magnetic field, followed by flow analysis. This technique allows us to characterize EVs populations according to their antigenic distribution, including minor EV fractions. We demonstrated that the individual blood EVs carry different sets of antigens, none being ubiquitous, and quantified their distribution. The physiological significance of antigenically different EVs and their correlation with different pathologies can now be directly addressed.

KW - Antigenic make-up

KW - Exosomes

KW - Extracellular vesicles

KW - Flow cytometry

KW - Microvesicles

UR - http://www.scopus.com/inward/record.url?scp=84933532643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84933532643&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2014.09.020

DO - 10.1016/j.nano.2014.09.020

M3 - Article

C2 - 25481806

AN - SCOPUS:84933532643

VL - 11

SP - 489

EP - 498

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

SN - 1549-9634

IS - 3

ER -