Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs

L. L. Green, M. C. Hardy, C. E. Maynard-Currie, H. Tsuda, D. M. Louie, M. J. Mendez, H. Abderrahim, M. Noguchi, D. H. Smith, Y. Zeng, N. E. David, H. Sasai, D. Garza, D. G. Brenner, J. F. Hales, R. P. McGuinness, D. J. Capon, S. Klapholz, A. Jakobovits

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

We describe a strategy for producing human monoclonal antibodies in mice by introducing large segments of the human heavy and κ light chain loci contained on yeast artificial chromosomes into the mouse germline. Such mice produce a diverse repertoire of human heavy and light chains, and upon immunization with tetanus toxin have been used to derive antigen-specific, fully human monoclonal antibodies. Breeding such animals with mice engineered by gene targeting to be deficient in mouse immunoglobulin (Ig) production has led to a mouse strain in which high levels of antibodies are produced, mostly comprised of both human heavy and light chains. These strains should provide insight into the adoptive human antibody response and permit the development of fully human monoclonal antibodies with therapeutic potential.

Original languageEnglish
Pages (from-to)13-21
Number of pages9
JournalNature Genetics
Volume7
Issue number1
DOIs
Publication statusPublished - 1 May 1994
Externally publishedYes

Fingerprint

Immunoglobulin Light Chains
Immunoglobulin Heavy Chains
Monoclonal Antibodies
Antigens
Light
Yeast Artificial Chromosomes
Tetanus Toxin
Gene Targeting
Human Development
Antibody Formation
Breeding
Immunoglobulins
Immunization
Antibodies

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Green, L. L., Hardy, M. C., Maynard-Currie, C. E., Tsuda, H., Louie, D. M., Mendez, M. J., ... Jakobovits, A. (1994). Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs. Nature Genetics, 7(1), 13-21. https://doi.org/10.1038/ng0594-13

Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs. / Green, L. L.; Hardy, M. C.; Maynard-Currie, C. E.; Tsuda, H.; Louie, D. M.; Mendez, M. J.; Abderrahim, H.; Noguchi, M.; Smith, D. H.; Zeng, Y.; David, N. E.; Sasai, H.; Garza, D.; Brenner, D. G.; Hales, J. F.; McGuinness, R. P.; Capon, D. J.; Klapholz, S.; Jakobovits, A.

In: Nature Genetics, Vol. 7, No. 1, 01.05.1994, p. 13-21.

Research output: Contribution to journalArticle

Green, LL, Hardy, MC, Maynard-Currie, CE, Tsuda, H, Louie, DM, Mendez, MJ, Abderrahim, H, Noguchi, M, Smith, DH, Zeng, Y, David, NE, Sasai, H, Garza, D, Brenner, DG, Hales, JF, McGuinness, RP, Capon, DJ, Klapholz, S & Jakobovits, A 1994, 'Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs', Nature Genetics, vol. 7, no. 1, pp. 13-21. https://doi.org/10.1038/ng0594-13
Green, L. L. ; Hardy, M. C. ; Maynard-Currie, C. E. ; Tsuda, H. ; Louie, D. M. ; Mendez, M. J. ; Abderrahim, H. ; Noguchi, M. ; Smith, D. H. ; Zeng, Y. ; David, N. E. ; Sasai, H. ; Garza, D. ; Brenner, D. G. ; Hales, J. F. ; McGuinness, R. P. ; Capon, D. J. ; Klapholz, S. ; Jakobovits, A. / Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs. In: Nature Genetics. 1994 ; Vol. 7, No. 1. pp. 13-21.
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