Anticancer activity of camel milk via induction of autophagic death in human colorectal and breast cancer cells

Roopesh Krishnankutty, Ahmad Iskandarani, Lubna Therachiyil, Shahab Uddin, Fouad Azizi, Michael Kulinski, Ajaz Ahmad Bhat, Ramzi M. Mohammad

Research output: Contribution to journalArticle

2 Citations (Scopus)


Background/ Objective: Camel milk is traditionally known for its human health benefits and believed to be a remedy for various human ailments including cancer. The study was aimed to evaluate the inhibitory effects of commercially available camel milk on cancer cells and its underlying mechanism(s). Materials and Methods: Two cell lines: colorectal cancer HCT 116 and breast cancer MCF-7 were cultured with different doses of camel milk. The effects of camel milk on cell death were determined by MTT assay, viability by trypan blue exclusion assay and migration by in vitro scratch assay. The mechanism was elucidated by western blotting and confocal microscopy was used to confirm autophagy. Results: Camel milk significantly reduced proliferation, viability as well as migration of both the cells. The accumulation of LC3-II protein along with reduction in expression of p62 and Atg 5-12, the autophagy proteins implied induction of autophagy. The (GFP)-LC3 puncta detected by confocal microscopy confirmed the autophagosome formation in response to camel milk treatment. Conclusion: Camel milk exerted antiproliferative effects on human colorectal HCT 116 and breast MCF-7 cancer cells by inducing autophagy.

Original languageEnglish
Pages (from-to)3501-3509
Number of pages9
JournalAsian Pacific Journal of Cancer Prevention
Issue number12
Publication statusPublished - 1 Dec 2018



  • Autophagy
  • Breast cancer
  • Camel milk
  • Colorectal cancer

ASJC Scopus subject areas

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

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