Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development

Emmanuel Labourier, Henri Marc Bourbon, Imed Gallouzi, Maggy Fostier, Eric Allemand, Jamal Tazi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Specific recognition of splice sites within metazoan mRNA precursors (pre-mRNAs) is a potential stage for gene regulation by alternative splicing. Splicing factors of the SR protein family play a major role in this regulation, as they are required for early recognition of splice sites during spliceosome assembly. Here, we describe the characterization of RSF1, a splicing repressor isolated from Drosophila, that functionally antagonizes SR proteins. Like the latter, RSF1 comprises an amino-terminal RRM-type RNA- binding domain, whereas its carboxy-terminal part is enriched in glycine (G), arginine (R), and serine (S) residues (GRS domain). RSF1 induces a dose- sensitive inhibition of splicing for several reporter pre-mRNAs, an inhibition that occurs at the level of early splicing complexes formation. RSF1 interacts, through its GRS domain, with the RS domain of the SR protein SF2/ASF and prevents the latter from cooperating with the U1 small nuclear ribonucleoprotein particle (U1 snRNP) in binding pre-mRNA. Furthermore, overproduction of RSF 1 in the fly rescues several developmental defects caused by overexpression of the splicing activator SR protein B52/SRp55. Therefore, RSF1 may correspond to the prototypical member of a novel family of general splicing repressors that selectively antagonize the effect of SR proteins on 5' splice-site recognition.

Original languageEnglish
Pages (from-to)740-753
Number of pages14
JournalGenes and Development
Volume13
Issue number6
Publication statusPublished - 15 Mar 1999
Externally publishedYes

Fingerprint

RNA Precursors
Drosophila
Proteins
U1 Small Nuclear Ribonucleoproteins
Small Nuclear Ribonucleoproteins
Spliceosomes
RNA Splice Sites
Alternative Splicing
Diptera
Glycine
Serine
Arginine
In Vitro Techniques
Genes

Keywords

  • B52/SRp55
  • pre-mRNA splicing
  • RNA-binding proteins
  • RSF1
  • SF2/ASF
  • U1 snRNP

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Labourier, E., Bourbon, H. M., Gallouzi, I., Fostier, M., Allemand, E., & Tazi, J. (1999). Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development. Genes and Development, 13(6), 740-753.

Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development. / Labourier, Emmanuel; Bourbon, Henri Marc; Gallouzi, Imed; Fostier, Maggy; Allemand, Eric; Tazi, Jamal.

In: Genes and Development, Vol. 13, No. 6, 15.03.1999, p. 740-753.

Research output: Contribution to journalArticle

Labourier, E, Bourbon, HM, Gallouzi, I, Fostier, M, Allemand, E & Tazi, J 1999, 'Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development', Genes and Development, vol. 13, no. 6, pp. 740-753.
Labourier, Emmanuel ; Bourbon, Henri Marc ; Gallouzi, Imed ; Fostier, Maggy ; Allemand, Eric ; Tazi, Jamal. / Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development. In: Genes and Development. 1999 ; Vol. 13, No. 6. pp. 740-753.
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