ANP32E is a histone chaperone that removes H2A.Z from chromatin

Arnaud Obri, Khalid Ouararhni, Christophe Papin, Marie Laure Diebold, Kiran Padmanabhan, Martin Marek, Isabelle Stoll, Ludovic Roy, Patrick T. Reilly, Tak W. Mak, Stefan Dimitrov, Christophe Romier, Ali Hamiche

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Abstract

H2A.Z is an essential histone variant implicated in the regulation of key nuclear events. However, the metazoan chaperones responsible for H2A.Z deposition and its removal from chromatin remain unknown. Here we report the identification and characterization of the human protein ANP32E as a specific H2A.Z chaperone. We show that ANP32E is a member of the presumed H2A.Z histone-exchange complex p400/TIP60. ANP32E interacts with a short region of the docking domain of H2A.Z through a new motif termed H2A.Z interacting domain (ZID). The 1.48 Å resolution crystal structure of the complex formed between the ANP32E-ZID and the H2A.Z/H2B dimer and biochemical data support an underlying molecular mechanism for H2A.Z/H2B eviction from the nucleosome and its stabilization by ANP32E through a specific extension of the H2A.Z carboxy-terminal α-helix. Finally, analysis of H2A.Z localization in ANP32E-/-cells by chromatin immunoprecipitation followed by sequencing shows genome-wide enrichment, redistribution and accumulation of H2A.Z at specific chromatin control regions, in particular at enhancers and insulators.

Original languageEnglish
Pages (from-to)648-653
Number of pages6
JournalNature
Volume505
Issue number7485
DOIs
Publication statusPublished - 2014
Externally publishedYes

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Histone Chaperones
Histones
Chromatin
Nucleosomes
Chromatin Immunoprecipitation
Genome

ASJC Scopus subject areas

  • General

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Obri, A., Ouararhni, K., Papin, C., Diebold, M. L., Padmanabhan, K., Marek, M., ... Hamiche, A. (2014). ANP32E is a histone chaperone that removes H2A.Z from chromatin. Nature, 505(7485), 648-653. https://doi.org/10.1038/nature12922

ANP32E is a histone chaperone that removes H2A.Z from chromatin. / Obri, Arnaud; Ouararhni, Khalid; Papin, Christophe; Diebold, Marie Laure; Padmanabhan, Kiran; Marek, Martin; Stoll, Isabelle; Roy, Ludovic; Reilly, Patrick T.; Mak, Tak W.; Dimitrov, Stefan; Romier, Christophe; Hamiche, Ali.

In: Nature, Vol. 505, No. 7485, 2014, p. 648-653.

Research output: Contribution to journalArticle

Obri, A, Ouararhni, K, Papin, C, Diebold, ML, Padmanabhan, K, Marek, M, Stoll, I, Roy, L, Reilly, PT, Mak, TW, Dimitrov, S, Romier, C & Hamiche, A 2014, 'ANP32E is a histone chaperone that removes H2A.Z from chromatin', Nature, vol. 505, no. 7485, pp. 648-653. https://doi.org/10.1038/nature12922
Obri A, Ouararhni K, Papin C, Diebold ML, Padmanabhan K, Marek M et al. ANP32E is a histone chaperone that removes H2A.Z from chromatin. Nature. 2014;505(7485):648-653. https://doi.org/10.1038/nature12922
Obri, Arnaud ; Ouararhni, Khalid ; Papin, Christophe ; Diebold, Marie Laure ; Padmanabhan, Kiran ; Marek, Martin ; Stoll, Isabelle ; Roy, Ludovic ; Reilly, Patrick T. ; Mak, Tak W. ; Dimitrov, Stefan ; Romier, Christophe ; Hamiche, Ali. / ANP32E is a histone chaperone that removes H2A.Z from chromatin. In: Nature. 2014 ; Vol. 505, No. 7485. pp. 648-653.
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