ANGPTL8/Betatrophin R59W variant is associated with higher glucose level in non-diabetic Arabs living in Kuwaits

Mohamed Abu-Farha, Motasem Melhem, Jehad Abubaker, Kazem Behbehani, Osama Alsmadi, Naser Elkum

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: ANGPTL8 (betatrophin) has been recently identified as a regulator of lipid metabolism through its interaction with ANGPTL3. A sequence variant in ANGPTL8 has been shown to associate with lower level of Low Density Lipoprotein (LDL) and High Density Lipoprotein (HDL). The objective of this study is to identify sequence variants in ANGPTL8 gene in Arabs and investigate their association with ANGPTL8 plasma level and clinical parameters. Methods: A cross sectional study was designed to examine the level of ANGPTL8 in 283 non-diabetic Arabs, and to identify its sequence variants using Sanger sequencing and their association with various clinical parameters. Results: Using Sanger sequencing, we sequenced the full ANGPTL8 gene in 283 Arabs identifying two single nucleotide polymorphisms (SNPs) Rs.892066 and Rs.2278426 in the coding region. Our data shows for the first time that Arabs with the heterozygote form of (c.194C > T Rs.2278426) had higher level of Fasting Blood Glucose (FBG) compared to the CC homozygotes. LDL and HDL level in these subjects did not show significant difference between the two subgroups. Circulation level of ANGPTL8 did not vary between the two forms. No significant changes were observed between the various forms of Rs.892066 variant and FBG, LDL or HDL. Conclusion: Our data shows for the first time that heterozygote form of ANGPTL8 Rs.2278426 variant was associated with higher FBG level in Arabs highlighting the importance of these variants in controlling the function of betatrophin.

Original languageEnglish
Article number195
JournalLipids in Health and Disease
Volume15
Issue number1
DOIs
Publication statusPublished - 11 Feb 2016

Fingerprint

Kuwait
HDL Lipoproteins
LDL Lipoproteins
Blood Glucose
Glucose
Genes
Fasting
Heterozygote
Polymorphism
Nucleotides
Plasmas
Homozygote
Lipid Metabolism
Single Nucleotide Polymorphism
Cross-Sectional Studies

Keywords

  • ANGPTL8
  • Glucose metabolism
  • HDL
  • LDL
  • Lipid metabolism
  • Sanger sequencing
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

ANGPTL8/Betatrophin R59W variant is associated with higher glucose level in non-diabetic Arabs living in Kuwaits. / Abu-Farha, Mohamed; Melhem, Motasem; Abubaker, Jehad; Behbehani, Kazem; Alsmadi, Osama; Elkum, Naser.

In: Lipids in Health and Disease, Vol. 15, No. 1, 195, 11.02.2016.

Research output: Contribution to journalArticle

Abu-Farha, Mohamed ; Melhem, Motasem ; Abubaker, Jehad ; Behbehani, Kazem ; Alsmadi, Osama ; Elkum, Naser. / ANGPTL8/Betatrophin R59W variant is associated with higher glucose level in non-diabetic Arabs living in Kuwaits. In: Lipids in Health and Disease. 2016 ; Vol. 15, No. 1.
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AU - Melhem, Motasem

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AU - Behbehani, Kazem

AU - Alsmadi, Osama

AU - Elkum, Naser

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AB - Background: ANGPTL8 (betatrophin) has been recently identified as a regulator of lipid metabolism through its interaction with ANGPTL3. A sequence variant in ANGPTL8 has been shown to associate with lower level of Low Density Lipoprotein (LDL) and High Density Lipoprotein (HDL). The objective of this study is to identify sequence variants in ANGPTL8 gene in Arabs and investigate their association with ANGPTL8 plasma level and clinical parameters. Methods: A cross sectional study was designed to examine the level of ANGPTL8 in 283 non-diabetic Arabs, and to identify its sequence variants using Sanger sequencing and their association with various clinical parameters. Results: Using Sanger sequencing, we sequenced the full ANGPTL8 gene in 283 Arabs identifying two single nucleotide polymorphisms (SNPs) Rs.892066 and Rs.2278426 in the coding region. Our data shows for the first time that Arabs with the heterozygote form of (c.194C > T Rs.2278426) had higher level of Fasting Blood Glucose (FBG) compared to the CC homozygotes. LDL and HDL level in these subjects did not show significant difference between the two subgroups. Circulation level of ANGPTL8 did not vary between the two forms. No significant changes were observed between the various forms of Rs.892066 variant and FBG, LDL or HDL. Conclusion: Our data shows for the first time that heterozygote form of ANGPTL8 Rs.2278426 variant was associated with higher FBG level in Arabs highlighting the importance of these variants in controlling the function of betatrophin.

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KW - Glucose metabolism

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KW - Lipid metabolism

KW - Sanger sequencing

KW - Single nucleotide polymorphism

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