Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly

Mohamed Emara, Pavel Ivanov, Tyler Hickman, Nemisha Dawra, Sarah Tisdale, Nancy Kedersha, Guo Fu Hu, Paul Anderson

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

Angiogenin (ANG) is a secreted ribonuclease that cleaves tRNA to initiate a stress-response program in mammalian cells. Here we show that ANG inhibits protein synthesis and promotes arsenite- and pateamine A-induced assembly of stress granules (SGs). These effects are abrogated in cells transfected with the ANG inhibitor RNH1. Transfection of natural or synthetic 5′-but not 3′-tRNA fragments (tRNA-derived stress-induced RNAs; tiRNAs) induces the phospho-eukaryotic translation initiation factor 2α-independent assembly of SGs. Natural 5′-tiRNAs but not 3′-tiRNAs are capped with a 5′-monophosphate that is required for optimal SG assembly. These findings reveal that SG assembly is a component of the ANG- and tiRNA-induced stress response program.

Original languageEnglish
Pages (from-to)10959-10968
Number of pages10
JournalJournal of Biological Chemistry
Volume285
Issue number14
DOIs
Publication statusPublished - 2 Apr 2010
Externally publishedYes

Fingerprint

Transfer RNA
RNA
Eukaryotic Initiation Factor-2
Eukaryotic Initiation Factors
Ribonucleases
Prokaryotic Initiation Factor-2
Transfection
angiogenin
Cells
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly. / Emara, Mohamed; Ivanov, Pavel; Hickman, Tyler; Dawra, Nemisha; Tisdale, Sarah; Kedersha, Nancy; Hu, Guo Fu; Anderson, Paul.

In: Journal of Biological Chemistry, Vol. 285, No. 14, 02.04.2010, p. 10959-10968.

Research output: Contribution to journalArticle

Emara, M, Ivanov, P, Hickman, T, Dawra, N, Tisdale, S, Kedersha, N, Hu, GF & Anderson, P 2010, 'Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly', Journal of Biological Chemistry, vol. 285, no. 14, pp. 10959-10968. https://doi.org/10.1074/jbc.M109.077560
Emara, Mohamed ; Ivanov, Pavel ; Hickman, Tyler ; Dawra, Nemisha ; Tisdale, Sarah ; Kedersha, Nancy ; Hu, Guo Fu ; Anderson, Paul. / Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 14. pp. 10959-10968.
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