Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly

Mohamed Emara, Pavel Ivanov, Tyler Hickman, Nemisha Dawra, Sarah Tisdale, Nancy Kedersha, Guo Fu Hu, Paul Anderson

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Abstract

Angiogenin (ANG) is a secreted ribonuclease that cleaves tRNA to initiate a stress-response program in mammalian cells. Here we show that ANG inhibits protein synthesis and promotes arsenite- and pateamine A-induced assembly of stress granules (SGs). These effects are abrogated in cells transfected with the ANG inhibitor RNH1. Transfection of natural or synthetic 5′-but not 3′-tRNA fragments (tRNA-derived stress-induced RNAs; tiRNAs) induces the phospho-eukaryotic translation initiation factor 2α-independent assembly of SGs. Natural 5′-tiRNAs but not 3′-tiRNAs are capped with a 5′-monophosphate that is required for optimal SG assembly. These findings reveal that SG assembly is a component of the ANG- and tiRNA-induced stress response program.

Original languageEnglish
Pages (from-to)10959-10968
Number of pages10
JournalJournal of Biological Chemistry
Volume285
Issue number14
DOIs
Publication statusPublished - 2 Apr 2010
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Emara, M., Ivanov, P., Hickman, T., Dawra, N., Tisdale, S., Kedersha, N., Hu, G. F., & Anderson, P. (2010). Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly. Journal of Biological Chemistry, 285(14), 10959-10968. https://doi.org/10.1074/jbc.M109.077560