Androstenedione and testosterone levels correlate with in vitro fertilization rates in insulin-resistant women

Thozhukat Sathyapalan, Emily H. Dickerson, Stephen M. Maguiness, John Robinson, Youssra Dakroury, Stephen Atkin

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Abstract

Background The free androgen index (FAI) calculated using total testosterone measured by immunoassay does not correlate with oocyte fertilization rates; however, there is considerable cross-reactivity for testosterone with other androgens by immunoassay that is not found using isotope dilution liquid chromatography–tandem mass spectrometry (LC/MS/MS). Aims This study was done to determine relationship between total testosterone and androstenedione measured by LC/MS/MS and fertilization rates. Study design 49 infertile women without polycystic ovary syndrome were recruited preceding an in vitro fertilization (IVF) cycle. Serum testosterone and androstenedione were measured by LC/MS/MS and correlated with IVF parameters non-insulin resistant compared with insulin resistant (homeostatic model assessment >2.5) women. Results For non-insulin resistant women, total testosterone FAI and androstenedione did not correlate with oocyte fertilization rates. In insulin-resistant women, there was a negative correlation between both testosterone and FAI and fertilization rates (r=−0.62, p<0.03) and r=−0.73, p=0.02, respectively). There was a positive correlation between androstenedione and fertilization rates (r=0.87, p<0.01). Conclusion For insulin-resistant women, increases in testosterone and FAI were associated with reduced fertilization rates and androstenedione was associated with increased fertilization rates when measured by LC/ MS/MS that should be considered to be the measurement method of choice.

Original languageEnglish
Article numbere000387
JournalBMJ Open Diabetes Research and Care
Volume5
Issue number1
DOIs
Publication statusPublished - 1 May 2017

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Androstenedione
Fertilization in Vitro
Fertilization
Testosterone
Insulin
Androgens
Immunoassay
Oocytes
Polycystic Ovary Syndrome
Isotopes
Mass Spectrometry
Serum

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

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Androstenedione and testosterone levels correlate with in vitro fertilization rates in insulin-resistant women. / Sathyapalan, Thozhukat; Dickerson, Emily H.; Maguiness, Stephen M.; Robinson, John; Dakroury, Youssra; Atkin, Stephen.

In: BMJ Open Diabetes Research and Care, Vol. 5, No. 1, e000387, 01.05.2017.

Research output: Contribution to journalArticle

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