Analysis of RBC-microparticles in stored whole blood bags – a promising marker to detect blood doping in sports?

Sven Christian Voss, Morana Jaganjac, Amna Mohamed Al-Thani, Jean-Charles B. Grivel, Christophe M. Raynaud, Hind Al-Jaber, Afnan Saleh Al-Menhali, Zeyed Ahmad Merenkov, Mohammed Alsayrafi, Aishah Latiff, Costas Georgakopoulos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Blood doping in sports is prohibited by the World Anti-Doping Agency (WADA). To find a possible biomarker for the detection of blood doping, we investigated the changes in blood stored in CPDA-1 blood bags of eight healthy subjects who donated one unit of blood. Aliquots were taken on days 0, 14, and 35. Platelet-free plasma was prepared and stored at −80°C until analysis on a flow cytometer dedicated for the analysis of microparticles (MPs). Changes in the number of red blood cell (RBC) -MPs were highly significant (p < 0.0001) with a mean of 219 (10^3/μL) on day 0 changing to 23 120 (10^3/μL) on day 14 and 29 310 (10^3/μL) on day 35. We conclude that RBC-MPs seem to be a promising biomarker for doping control but confirmation by a transfusion study is necessary.

Original languageEnglish
Pages (from-to)1794-1798
Number of pages5
JournalDrug Testing and Analysis
Volume9
Issue number11-12
DOIs
Publication statusPublished - 1 Nov 2017

    Fingerprint

Keywords

  • autologous
  • blood
  • doping
  • microparticles
  • transfusion

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Pharmaceutical Science
  • Spectroscopy

Cite this

Voss, S. C., Jaganjac, M., Al-Thani, A. M., Grivel, J-C. B., Raynaud, C. M., Al-Jaber, H., Al-Menhali, A. S., Merenkov, Z. A., Alsayrafi, M., Latiff, A., & Georgakopoulos, C. (2017). Analysis of RBC-microparticles in stored whole blood bags – a promising marker to detect blood doping in sports? Drug Testing and Analysis, 9(11-12), 1794-1798. https://doi.org/10.1002/dta.2212