Analysis of RBC-microparticles in stored whole blood bags – a promising marker to detect blood doping in sports?

Sven Christian Voss, Morana Jaganjac, Amna Mohamed Al-Thani, Jean-Charles B. Grivel, Christophe M. Raynaud, Hind Al-Jaber, Afnan Saleh Al-Menhali, Zeyed Ahmad Merenkov, Mohammed Alsayrafi, Aishah Latiff, Costas Georgakopoulos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Blood doping in sports is prohibited by the World Anti-Doping Agency (WADA). To find a possible biomarker for the detection of blood doping, we investigated the changes in blood stored in CPDA-1 blood bags of eight healthy subjects who donated one unit of blood. Aliquots were taken on days 0, 14, and 35. Platelet-free plasma was prepared and stored at −80°C until analysis on a flow cytometer dedicated for the analysis of microparticles (MPs). Changes in the number of red blood cell (RBC) -MPs were highly significant (p < 0.0001) with a mean of 219 (10^3/μL) on day 0 changing to 23 120 (10^3/μL) on day 14 and 29 310 (10^3/μL) on day 35. We conclude that RBC-MPs seem to be a promising biomarker for doping control but confirmation by a transfusion study is necessary.

Original languageEnglish
Pages (from-to)1794-1798
Number of pages5
JournalDrug Testing and Analysis
Volume9
Issue number11-12
DOIs
Publication statusPublished - 1 Nov 2017

Fingerprint

Doping in Sports
Sports
sport
Blood
Erythrocytes
blood
Doping (additives)
Biomarkers
Healthy Volunteers
Blood Platelets
biomarker
Platelets
analysis
marker
Cells
Plasmas
plasma

Keywords

  • autologous
  • blood
  • doping
  • microparticles
  • transfusion

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Pharmaceutical Science
  • Spectroscopy

Cite this

Analysis of RBC-microparticles in stored whole blood bags – a promising marker to detect blood doping in sports? / Voss, Sven Christian; Jaganjac, Morana; Al-Thani, Amna Mohamed; Grivel, Jean-Charles B.; Raynaud, Christophe M.; Al-Jaber, Hind; Al-Menhali, Afnan Saleh; Merenkov, Zeyed Ahmad; Alsayrafi, Mohammed; Latiff, Aishah; Georgakopoulos, Costas.

In: Drug Testing and Analysis, Vol. 9, No. 11-12, 01.11.2017, p. 1794-1798.

Research output: Contribution to journalArticle

Voss, SC, Jaganjac, M, Al-Thani, AM, Grivel, J-CB, Raynaud, CM, Al-Jaber, H, Al-Menhali, AS, Merenkov, ZA, Alsayrafi, M, Latiff, A & Georgakopoulos, C 2017, 'Analysis of RBC-microparticles in stored whole blood bags – a promising marker to detect blood doping in sports?', Drug Testing and Analysis, vol. 9, no. 11-12, pp. 1794-1798. https://doi.org/10.1002/dta.2212
Voss, Sven Christian ; Jaganjac, Morana ; Al-Thani, Amna Mohamed ; Grivel, Jean-Charles B. ; Raynaud, Christophe M. ; Al-Jaber, Hind ; Al-Menhali, Afnan Saleh ; Merenkov, Zeyed Ahmad ; Alsayrafi, Mohammed ; Latiff, Aishah ; Georgakopoulos, Costas. / Analysis of RBC-microparticles in stored whole blood bags – a promising marker to detect blood doping in sports?. In: Drug Testing and Analysis. 2017 ; Vol. 9, No. 11-12. pp. 1794-1798.
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