Analysis of intimal proteoglycans in atherosclerosis-prone and atherosclerosis-resistant human arteries by mass spectrometry

Paul Talusan, Shahinaz Bedri, Suping Yang, Taj Kattapuram, Nilsa Silva, Peter J. Roughley, James R. Stone

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The propensity to develop atherosclerosis varies markedly among different sites in the human vasculature. To determine a possible cause for such differences in atherosclerosis susceptibility, a proteomics-based approach was used to assess the extracellular proteoglycan core protein composition of intimal hyperplasia from both the atherosclerosis-prone internal carotid artery and the atherosclerosis-resistant internal thoracic artery. The intimal proteoglycan composition in these preatherosclerotic lesions was found to be more complex than previously appreciated with up to eight distinct core proteins present, including the large extracellular proteoglycans versican and aggrecan, the basement membrane proteoglycan perlecan, the class I small leucine-rich proteoglycans biglycan and decorin, and the class II small leucine-rich proteoglycans lumican, fibromodulin, and prolargin/PRELP (proline arginine-rich end leucine-rich repeat protein). Although most of these proteoglycans seem to be present in similar amounts at the two locations, there was a selective enhanced deposition of lumican in the intima of the atherosclerosis-prone internal carotid artery compared with the intima of the atherosclerosis-resistant internal thoracic artery. The enhanced deposition of lumican in the intima of an atherosclerosis prone artery has important implications for the pathogenesis of atherosclerosis.

Original languageEnglish
Pages (from-to)1350-1357
Number of pages8
JournalMolecular and Cellular Proteomics
Volume4
Issue number9
DOIs
Publication statusPublished - Sep 2005
Externally publishedYes

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Tunica Intima
Proteoglycans
Mass spectrometry
Mass Spectrometry
Atherosclerosis
Arteries
Mammary Arteries
Internal Carotid Artery
Leucine
Versicans
Biglycan
Decorin
Aggrecans
Carotid Artery Diseases
Chemical analysis
Basement Membrane
Proline
Proteomics
Hyperplasia
Arginine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Analysis of intimal proteoglycans in atherosclerosis-prone and atherosclerosis-resistant human arteries by mass spectrometry. / Talusan, Paul; Bedri, Shahinaz; Yang, Suping; Kattapuram, Taj; Silva, Nilsa; Roughley, Peter J.; Stone, James R.

In: Molecular and Cellular Proteomics, Vol. 4, No. 9, 09.2005, p. 1350-1357.

Research output: Contribution to journalArticle

Talusan, Paul ; Bedri, Shahinaz ; Yang, Suping ; Kattapuram, Taj ; Silva, Nilsa ; Roughley, Peter J. ; Stone, James R. / Analysis of intimal proteoglycans in atherosclerosis-prone and atherosclerosis-resistant human arteries by mass spectrometry. In: Molecular and Cellular Proteomics. 2005 ; Vol. 4, No. 9. pp. 1350-1357.
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