An N-methyl-d-aspartate-receptor dependent, late-phase long-term depression in middle-aged mice identifies no GluN2-subunit bias

Tariq Ahmed, V. Sabanov, R. D'Hooge, D. Balschun

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14 Citations (Scopus)

Abstract

Late-phase long-term depression (L-LTD) in middle-aged mice has been difficult to achieve and maintain. Here we report an electrically induced, homosynaptic, input-specific form of LTD that could be stably maintained for at least 4 h in the CA1 area of hippocampal slices of 10-14 months old mice. This form of L-LTD was similar in magnitude in aged, middle-aged and young mice and was blocked by high concentrations of broad-spectrum N-methyl-d-aspartate receptor (NMDAR) antagonists such as d(-)-2-amino-5-phospho-pentanoic acid (d-AP5) and (R)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP). Extracellular and whole cell recordings revealed a decreased sensitivity to d-AP5 with age, without any differences in NMDAR conductance between the age groups tested. This L-LTD could be inhibited neither by common doses of NMDA-subunit specific antagonists like zinc, ifenprodil and Ro-25-6981, nor by various co-applications of these compounds. In addition to the lack of any GluN2 subunit bias, L-LTD did not show any discernible involvement of L-type voltage-gated calcium channels. In conclusion, our results do not support any specific role of NMDAR subunits in LTD.

Original languageEnglish
Pages (from-to)27-38
Number of pages12
JournalNeuroscience
Volume185
DOIs
Publication statusPublished - 30 Jun 2011
Externally publishedYes

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Keywords

  • Hippocampal cA1-region
  • In vitro
  • Long term depression
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

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