An in-vitro model of cardiac fibrillation with different degrees of complexity

Andreu M. Climent, M. S. Guillem, P. Lee, C. Bollensdorff, F. Atienza, M. E. Fernández-Santos, R. Sanz-Ruiz, P. L. Sánchez, F. Fernández-Avilés

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The difficulty in obtaining research models of chronic atrial fibrillation (AF) is one of the main constraints to make progress in the elucidation of the mechanisms of perpetuation of this arrhythmia. The aim of this study is to present a new model of in vitro AF with different degrees of complexity similar to those observed during AF with varying degrees of structural remodeling. In the present study, optical calcium mapping is used to characterize the degree of disorganization of the reentrant activity in HL-1 confluent cell cultures. Specifically, the number of simultaneous rotors is linked with the time and degree of inhomogeneity of cell cultures. HL-1 cell cultures showed electrophysiological characteristics dependent on their degree of confluency resembling processes with varying degrees of complexity as it occurs during AF with varying degrees of structural remodeling. This model could be useful for studying the effect of remodeling on the fibrillatory process and to evaluate new antiarrhythmic drugs.

    Original languageEnglish
    Title of host publicationIFMBE Proceedings
    Pages340-343
    Number of pages4
    Volume41
    DOIs
    Publication statusPublished - 2 Jan 2014
    Event13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013, MEDICON 2013 - Seville, Spain
    Duration: 25 Sep 201328 Sep 2013

    Other

    Other13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013, MEDICON 2013
    CountrySpain
    CitySeville
    Period25/9/1328/9/13

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    Keywords

    • Atrial fibrillation
    • Electrophysiology
    • HL-1 cells
    • Optical mapping

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Bioengineering

    Cite this

    Climent, A. M., Guillem, M. S., Lee, P., Bollensdorff, C., Atienza, F., Fernández-Santos, M. E., Sanz-Ruiz, R., Sánchez, P. L., & Fernández-Avilés, F. (2014). An in-vitro model of cardiac fibrillation with different degrees of complexity. In IFMBE Proceedings (Vol. 41, pp. 340-343) https://doi.org/10.1007/978-3-319-00846-2_84