An analytical approach to the preparation and characterization of subcellular membranes from canine mesenteric arteries

C. Y. Kwan, Christopher Triggle, A. K. Grover, R. M K W Lee, E. E. Daniel

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Subcellular membrane fractions were isolated ‘from dog mesenteric arteries by differential and isopynic sucrose density gradient centrifugations. Isolated membrane fractions were characterized by marker enzyme activities, morphological features and sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns. Our results show that the microsomal fraction isolated by conventional differential centrifugation was highly heterogenous and contained substantial amount of plasma membranes which could be further enriched as a light density membrane fraction on a discontinuous sucrose density gradient. The microsomal fraction and its subfractions were vesicular in appearance under electron microscope and were capable of binding and actively transporting Ca2+. The binding of Ca2+ and ATP-supported Ca2+-transport in the presence or absence of oxalate paralleled the distribution of plasma membrane marker enzyme activities suggesting that plasma membranes in vascular smooth muscle may play a major role in handling Ca2+ and thus the control of contractile function.

Original languageEnglish
Pages (from-to)275-314
Number of pages40
JournalPreparative Biochemistry
Volume13
Issue number4
DOIs
Publication statusPublished - 1 Nov 1983
Externally publishedYes

Fingerprint

Mesenteric Arteries
Cell membranes
Canidae
Centrifugation
Cell Membrane
Enzyme activity
Membranes
Sucrose
Subcellular Fractions
Oxalates
Density Gradient Centrifugation
Enzymes
Vascular Smooth Muscle
Sodium Dodecyl Sulfate
Muscle
Electron microscopes
Adenosine Triphosphate
Dogs
Electrons
Light

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

An analytical approach to the preparation and characterization of subcellular membranes from canine mesenteric arteries. / Kwan, C. Y.; Triggle, Christopher; Grover, A. K.; Lee, R. M K W; Daniel, E. E.

In: Preparative Biochemistry, Vol. 13, No. 4, 01.11.1983, p. 275-314.

Research output: Contribution to journalArticle

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