Amino acid sequence of the variable domains of a human anti-Rh(c) antibody

Presence of an unusually long CDR3 in the λ chain

Lotfi Chouchane, J. L. Guillaume, D. Goossens, Ph Rouger, A. Donny Strosberg

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The complete amino acid sequence of the λ light chain and the variable domain of the heavy chain of an anti-Rh(c) human monoclonal antibody were determined. The λ chain presents a long third complementarity-determining region sequence with unusual amino acid insertions at the C-terminus. The proposed sequence indicates that this λ chain may be assigned to the variable region subgroup I. The J segment is identical to that of Jλ2 except for the first amino acid residue. Positions 152 (serine) and 190 (arginine) from this sequence correspond to the Kern-Oz- isotype, respectively. The VH segment can be classified as a VHIII subgroup member. The CDR1 segment of the anti-Rh(c) VH region has the same sequence as the VH of human BRO protein except for the first residue of the CDR1. The amino acid sequence of the anti-Rh(c) D segment does not match any published D segment. The JH segment used in this protein can be classified as a JH3 with a single amino acid difference at the fourth residue.

Original languageEnglish
Pages (from-to)1179-1186
Number of pages8
JournalMolecular Immunology
Volume26
Issue number12
DOIs
Publication statusPublished - 1989
Externally publishedYes

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Amino Acid Sequence
Amino Acids
Complementarity Determining Regions
Serine
Arginine
Proteins
Monoclonal Antibodies
Light
anti-c antibody

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

Cite this

Amino acid sequence of the variable domains of a human anti-Rh(c) antibody : Presence of an unusually long CDR3 in the λ chain. / Chouchane, Lotfi; Guillaume, J. L.; Goossens, D.; Rouger, Ph; Strosberg, A. Donny.

In: Molecular Immunology, Vol. 26, No. 12, 1989, p. 1179-1186.

Research output: Contribution to journalArticle

Chouchane, Lotfi ; Guillaume, J. L. ; Goossens, D. ; Rouger, Ph ; Strosberg, A. Donny. / Amino acid sequence of the variable domains of a human anti-Rh(c) antibody : Presence of an unusually long CDR3 in the λ chain. In: Molecular Immunology. 1989 ; Vol. 26, No. 12. pp. 1179-1186.
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