Theactions of aluminium on glutamate-activated currents of acutely isolated hippocampal neurones were investigated. TV-methyl-D-aspartate (NMDA), a-amino- 3-hydroxy-5-methylisoxazole-4-proprionic acid (AMPA) and glutamate mediated currents were reduced by 50% in the presence of 1.4µg ml-1 aluminium. Higher concentrations (≥ 2.7 µg ml-1) inhibited all currents completely and irreversibly. Additionally, successive application of agonists in the presence of 2.7 µg ml'1 aluminium resulted in non-specific membrane currents followed by the loss of the seal resistance. Application of aluminium perse had no influence on resting membrane current or voltage-activated sodium currents. The estimation of the concen- tration-response relationship of the action of aluminium on NMDA-activated currents revealed a threshold concentration < 0.27 µg mb-1. Our data indicate that glutamate receptors are putative sites of action in aluminium neurotoxicity.
|Number of pages||4|
|Publication status||Published - 1994|
- Glutamate-activated channels
- Hippocampal neurons
ASJC Scopus subject areas