Altered structure and expression of RB1 gene and increased phosphorylation of pRb in human vestibular schwannomas

Remy Thomas, P. D.Antony Herold Prabhu, J. Mathivanan, Rohini, D. Sivakumar, P. N. Jayakumar, B. Indira Devi, S. Satish, K. V.R. Sastry, R. Gope

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Tumor-specific alterations at the RB1 gene locus in 30 human vestibular schwannomas including 10 NF2 and 20 sporadic cases were analysed. Southern blot analysis of DNA from these samples revealed loss of heterozygosity (LOH) at the RB1 locus in 6 of 24 informative cases (25%) compared to normal blood DNAs from the same patients. Northern blot analysis showed normal size RB1 mRNA in all the tumor samples. However, there was a 2-5-fold increase in the level of expression of the RB1 gene in all the tumor samples compared to the WI38 cell line which was used as control. Western blot analysis of the RB1 protein, pRb showed a 2.5-5-fold increase in the level of total pRb as compared to normal WI38 cell line. Sixty five to seventy five percent of the total pRb were in phosphorylated form in most tumors. The LOH at the RB1 gene locus suggests genetic instability in these patients. Further, increased levels of RB1 mRNA, total pRb and the phosphorylated form of pRb suggests that RB1gene in these tumors may have anti-apoptotic function. These results suggest that the RB1 gene has a major role in the development of human vestibular schwannomas.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume271
Issue number1-2
DOIs
Publication statusPublished - 1 Mar 2005
Externally publishedYes

Fingerprint

Phosphorylation
Acoustic Neuroma
Tumors
Genes
Gene Expression
Loss of Heterozygosity
Neoplasms
Cells
Cell Line
Messenger RNA
Genetic Loci
DNA
Human Development
Southern Blotting
Northern Blotting
Blood
Western Blotting
Proteins

Keywords

  • Gene expression
  • Gene structure
  • Human retinoblastoma gene (RB1)
  • Human vestibular schwannomas
  • pRb
  • pRb phosphorylation
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Altered structure and expression of RB1 gene and increased phosphorylation of pRb in human vestibular schwannomas. / Thomas, Remy; Prabhu, P. D.Antony Herold; Mathivanan, J.; Rohini; Sivakumar, D.; Jayakumar, P. N.; Devi, B. Indira; Satish, S.; Sastry, K. V.R.; Gope, R.

In: Molecular and Cellular Biochemistry, Vol. 271, No. 1-2, 01.03.2005, p. 113-121.

Research output: Contribution to journalArticle

Thomas, R, Prabhu, PDAH, Mathivanan, J, Rohini, Sivakumar, D, Jayakumar, PN, Devi, BI, Satish, S, Sastry, KVR & Gope, R 2005, 'Altered structure and expression of RB1 gene and increased phosphorylation of pRb in human vestibular schwannomas', Molecular and Cellular Biochemistry, vol. 271, no. 1-2, pp. 113-121. https://doi.org/10.1007/s11010-005-5617-0
Thomas, Remy ; Prabhu, P. D.Antony Herold ; Mathivanan, J. ; Rohini ; Sivakumar, D. ; Jayakumar, P. N. ; Devi, B. Indira ; Satish, S. ; Sastry, K. V.R. ; Gope, R. / Altered structure and expression of RB1 gene and increased phosphorylation of pRb in human vestibular schwannomas. In: Molecular and Cellular Biochemistry. 2005 ; Vol. 271, No. 1-2. pp. 113-121.
@article{a3d33e2a8a1045a194724a20e6181ea3,
title = "Altered structure and expression of RB1 gene and increased phosphorylation of pRb in human vestibular schwannomas",
abstract = "Tumor-specific alterations at the RB1 gene locus in 30 human vestibular schwannomas including 10 NF2 and 20 sporadic cases were analysed. Southern blot analysis of DNA from these samples revealed loss of heterozygosity (LOH) at the RB1 locus in 6 of 24 informative cases (25{\%}) compared to normal blood DNAs from the same patients. Northern blot analysis showed normal size RB1 mRNA in all the tumor samples. However, there was a 2-5-fold increase in the level of expression of the RB1 gene in all the tumor samples compared to the WI38 cell line which was used as control. Western blot analysis of the RB1 protein, pRb showed a 2.5-5-fold increase in the level of total pRb as compared to normal WI38 cell line. Sixty five to seventy five percent of the total pRb were in phosphorylated form in most tumors. The LOH at the RB1 gene locus suggests genetic instability in these patients. Further, increased levels of RB1 mRNA, total pRb and the phosphorylated form of pRb suggests that RB1gene in these tumors may have anti-apoptotic function. These results suggest that the RB1 gene has a major role in the development of human vestibular schwannomas.",
keywords = "Gene expression, Gene structure, Human retinoblastoma gene (RB1), Human vestibular schwannomas, pRb, pRb phosphorylation, Tumor suppressor gene",
author = "Remy Thomas and Prabhu, {P. D.Antony Herold} and J. Mathivanan and Rohini and D. Sivakumar and Jayakumar, {P. N.} and Devi, {B. Indira} and S. Satish and Sastry, {K. V.R.} and R. Gope",
year = "2005",
month = "3",
day = "1",
doi = "10.1007/s11010-005-5617-0",
language = "English",
volume = "271",
pages = "113--121",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Altered structure and expression of RB1 gene and increased phosphorylation of pRb in human vestibular schwannomas

AU - Thomas, Remy

AU - Prabhu, P. D.Antony Herold

AU - Mathivanan, J.

AU - Rohini,

AU - Sivakumar, D.

AU - Jayakumar, P. N.

AU - Devi, B. Indira

AU - Satish, S.

AU - Sastry, K. V.R.

AU - Gope, R.

PY - 2005/3/1

Y1 - 2005/3/1

N2 - Tumor-specific alterations at the RB1 gene locus in 30 human vestibular schwannomas including 10 NF2 and 20 sporadic cases were analysed. Southern blot analysis of DNA from these samples revealed loss of heterozygosity (LOH) at the RB1 locus in 6 of 24 informative cases (25%) compared to normal blood DNAs from the same patients. Northern blot analysis showed normal size RB1 mRNA in all the tumor samples. However, there was a 2-5-fold increase in the level of expression of the RB1 gene in all the tumor samples compared to the WI38 cell line which was used as control. Western blot analysis of the RB1 protein, pRb showed a 2.5-5-fold increase in the level of total pRb as compared to normal WI38 cell line. Sixty five to seventy five percent of the total pRb were in phosphorylated form in most tumors. The LOH at the RB1 gene locus suggests genetic instability in these patients. Further, increased levels of RB1 mRNA, total pRb and the phosphorylated form of pRb suggests that RB1gene in these tumors may have anti-apoptotic function. These results suggest that the RB1 gene has a major role in the development of human vestibular schwannomas.

AB - Tumor-specific alterations at the RB1 gene locus in 30 human vestibular schwannomas including 10 NF2 and 20 sporadic cases were analysed. Southern blot analysis of DNA from these samples revealed loss of heterozygosity (LOH) at the RB1 locus in 6 of 24 informative cases (25%) compared to normal blood DNAs from the same patients. Northern blot analysis showed normal size RB1 mRNA in all the tumor samples. However, there was a 2-5-fold increase in the level of expression of the RB1 gene in all the tumor samples compared to the WI38 cell line which was used as control. Western blot analysis of the RB1 protein, pRb showed a 2.5-5-fold increase in the level of total pRb as compared to normal WI38 cell line. Sixty five to seventy five percent of the total pRb were in phosphorylated form in most tumors. The LOH at the RB1 gene locus suggests genetic instability in these patients. Further, increased levels of RB1 mRNA, total pRb and the phosphorylated form of pRb suggests that RB1gene in these tumors may have anti-apoptotic function. These results suggest that the RB1 gene has a major role in the development of human vestibular schwannomas.

KW - Gene expression

KW - Gene structure

KW - Human retinoblastoma gene (RB1)

KW - Human vestibular schwannomas

KW - pRb

KW - pRb phosphorylation

KW - Tumor suppressor gene

UR - http://www.scopus.com/inward/record.url?scp=20244368347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20244368347&partnerID=8YFLogxK

U2 - 10.1007/s11010-005-5617-0

DO - 10.1007/s11010-005-5617-0

M3 - Article

C2 - 15881662

AN - SCOPUS:20244368347

VL - 271

SP - 113

EP - 121

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -