Aerobic biofilm reactor for treating a commercial formulation of the herbicides 2,4-D and dicamba

Biodegradation kinetics and biofilm bacterial diversity

Saúl González-Cuna, Juvencio Galíndez-Mayer, Nora Ruiz-Ordaz, Selvasankar Murugesan, Alberto Piña-Escobedo, Jaime García-Mena, Emanuel Lima-Martínez, Fortunata Santoyo-Tepole

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A microbial community, able to use 2,4-D (2,4-dichlorophenoxyacetic acid) and dicamba (3,6-dichloro-2-methoxybenzoic acid) as carbon and energy sources, was selected in a continuously operated packed-bed column (PBC). Both active compounds are present in the Banvel herbicide. The predominant cultivable microorganisms isolated belong to the genera Burkholderia, Dyella, Mycobacterium, and Microbacterium. The presence of the catabolic genes tfdACD encoding enzymes involved in the 2,4-D metabolic pathway was evaluated for the bacterial isolates, with positive results for all of them. To evaluate the ability of the entire microbial community to degrade these herbicides in aerobic conditions a horizontal multistage-packed-bed reactor (HMPBR) was constructed. The reactor has four zones packed with fragments of volcanic rock, interspaced with four unpacked aerated zones. The continuous reactor, inoculated with the isolated microbial consortium, was run at increasing Banvel loading rates always operating in an aerobic environment and under steady-state conditions. In no case, 2,4-D or its main aromatic derivatives were detected in reactor's effluent, while dicamba was substantially degraded only at low loading rates. The highest degradation efficiencies of the herbicides were 100% for 2,4-D and 84% for dicamba. COD determinations showed that other components present in the commercial formulation of the herbicide were also removed. To evaluate changes in the diversity of the microbial consortium, massive semiconductor sequencing of 16S rDNA libraries was made. A remarkable variation in the bacterial diversity at phylum level, was observed in biofilm samples obtained from distinct packed bed zones of the reactor, and at different operating conditions.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalInternational Biodeterioration and Biodegradation
Volume107
DOIs
Publication statusPublished - 1 Feb 2016
Externally publishedYes

Fingerprint

Dicamba
2,4-Dichlorophenoxyacetic Acid
Herbicides
2,4 dichlorophenoxyacetic acid
Biofilms
Biodegradation
biofilm
herbicide
biodegradation
Packed beds
kinetics
Kinetics
Microbial Consortia
microbial community
Volcanic rocks
Gene encoding
Acids
Burkholderia
Semiconductors
Microorganisms

Keywords

  • 2,4-D
  • Biofilm reactor
  • Dicamba
  • Ion semiconductor sequencing
  • Microbial consortium
  • Pressure selection

ASJC Scopus subject areas

  • Microbiology
  • Biomaterials
  • Waste Management and Disposal

Cite this

Aerobic biofilm reactor for treating a commercial formulation of the herbicides 2,4-D and dicamba : Biodegradation kinetics and biofilm bacterial diversity. / González-Cuna, Saúl; Galíndez-Mayer, Juvencio; Ruiz-Ordaz, Nora; Murugesan, Selvasankar; Piña-Escobedo, Alberto; García-Mena, Jaime; Lima-Martínez, Emanuel; Santoyo-Tepole, Fortunata.

In: International Biodeterioration and Biodegradation, Vol. 107, 01.02.2016, p. 123-131.

Research output: Contribution to journalArticle

González-Cuna, Saúl ; Galíndez-Mayer, Juvencio ; Ruiz-Ordaz, Nora ; Murugesan, Selvasankar ; Piña-Escobedo, Alberto ; García-Mena, Jaime ; Lima-Martínez, Emanuel ; Santoyo-Tepole, Fortunata. / Aerobic biofilm reactor for treating a commercial formulation of the herbicides 2,4-D and dicamba : Biodegradation kinetics and biofilm bacterial diversity. In: International Biodeterioration and Biodegradation. 2016 ; Vol. 107. pp. 123-131.
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abstract = "A microbial community, able to use 2,4-D (2,4-dichlorophenoxyacetic acid) and dicamba (3,6-dichloro-2-methoxybenzoic acid) as carbon and energy sources, was selected in a continuously operated packed-bed column (PBC). Both active compounds are present in the Banvel herbicide. The predominant cultivable microorganisms isolated belong to the genera Burkholderia, Dyella, Mycobacterium, and Microbacterium. The presence of the catabolic genes tfdACD encoding enzymes involved in the 2,4-D metabolic pathway was evaluated for the bacterial isolates, with positive results for all of them. To evaluate the ability of the entire microbial community to degrade these herbicides in aerobic conditions a horizontal multistage-packed-bed reactor (HMPBR) was constructed. The reactor has four zones packed with fragments of volcanic rock, interspaced with four unpacked aerated zones. The continuous reactor, inoculated with the isolated microbial consortium, was run at increasing Banvel loading rates always operating in an aerobic environment and under steady-state conditions. In no case, 2,4-D or its main aromatic derivatives were detected in reactor's effluent, while dicamba was substantially degraded only at low loading rates. The highest degradation efficiencies of the herbicides were 100{\%} for 2,4-D and 84{\%} for dicamba. COD determinations showed that other components present in the commercial formulation of the herbicide were also removed. To evaluate changes in the diversity of the microbial consortium, massive semiconductor sequencing of 16S rDNA libraries was made. A remarkable variation in the bacterial diversity at phylum level, was observed in biofilm samples obtained from distinct packed bed zones of the reactor, and at different operating conditions.",
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AU - Piña-Escobedo, Alberto

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