Advantages of mRNA amplification for microarray analysis

A. L. Feldman, N. G. Costouros, E. Wang, M. Qian, F. M. Marincola, H. R. Alexander, Steven K. Libutti

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Expanding applications of cDNA microarrays such as fine needle aspiration biopsy and laser capture microdissection necessitate the ability to perform arrays with minute starting amounts of RNA. While methods for amplifying RNA have been advocated, the fidelity of array results using amplified material has not been fully validated. Here we demonstrate preserved fidelity in arrays using one or two rounds of mRNA amplification, validated by downstream real-time quantitative PCR. In addition, the quality of the array data was superior to that obtained using total RNA. Based on these results, we recommend routine mRNA amplification for all cDNA microarray-based analysis of gene expression.

Original languageEnglish
Pages (from-to)906-914
Number of pages9
JournalBioTechniques
Volume33
Issue number4
Publication statusPublished - 1 Oct 2002
Externally publishedYes

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Microarray Analysis
Microarrays
Amplification
RNA
Oligonucleotide Array Sequence Analysis
Messenger RNA
Microdissection
Complementary DNA
Laser Capture Microdissection
Biopsy
Fine Needle Biopsy
Gene expression
Needles
Real-Time Polymerase Chain Reaction
Gene Expression
Lasers

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Feldman, A. L., Costouros, N. G., Wang, E., Qian, M., Marincola, F. M., Alexander, H. R., & Libutti, S. K. (2002). Advantages of mRNA amplification for microarray analysis. BioTechniques, 33(4), 906-914.

Advantages of mRNA amplification for microarray analysis. / Feldman, A. L.; Costouros, N. G.; Wang, E.; Qian, M.; Marincola, F. M.; Alexander, H. R.; Libutti, Steven K.

In: BioTechniques, Vol. 33, No. 4, 01.10.2002, p. 906-914.

Research output: Contribution to journalArticle

Feldman, AL, Costouros, NG, Wang, E, Qian, M, Marincola, FM, Alexander, HR & Libutti, SK 2002, 'Advantages of mRNA amplification for microarray analysis', BioTechniques, vol. 33, no. 4, pp. 906-914.
Feldman AL, Costouros NG, Wang E, Qian M, Marincola FM, Alexander HR et al. Advantages of mRNA amplification for microarray analysis. BioTechniques. 2002 Oct 1;33(4):906-914.
Feldman, A. L. ; Costouros, N. G. ; Wang, E. ; Qian, M. ; Marincola, F. M. ; Alexander, H. R. ; Libutti, Steven K. / Advantages of mRNA amplification for microarray analysis. In: BioTechniques. 2002 ; Vol. 33, No. 4. pp. 906-914.
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