Adult cardiomyocytes express functional high-affinity receptors for basic fibroblast growth factor

L. Liu, K. B.S. Pasumarthi, R. R. Padua, Hamid Massaeli, R. R. Fandrich, G. N. Pierce, P. A. Cattini, E. Kardami

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

As a first step in addressing the question of function for basic fibroblast growth factor (bFGF) in the adult myocardium, expression of bFGF receptors by adult rat myocytes was investigated. Cross-linking of 125I- labeled bFGF to purified sarcolemmal vesicles from adult hearts indicated specific binding to 90- to 104-kDa proteins, whereas equilibrium binding studies revealed the presence of 'low'-affinity (1 nM) and 'high'-affinity (115 pM) sites. Adult myocytes were found to express short and long variants of bFGF receptor 1 (FGFR-1, tyrosine kinase) mRNA. Adult heart overall levels of FGFR-1 mRNA were decreased by about one-third of corresponding fetal values. Several lines of evidence indicated that bFGF receptors in adult cardiomyocytes in situ and/or in isolation are functional. Isolated adult myocytes were found to be capable of heparin-resistant internalization of 125I-labeled bFGF, to lose their viability after interaction with bFGF- saporin (a mitotoxin known to kill cells after entry via the bFGF receptor), and to respond to bFGF by activation of mitogen-activated protein kinase. In addition, introduction of exogenous bFGF into the myocardium by Langendorff perfusion resulted in stimulation of tyrosine phosphorylation in association with cardiomyocyte intercalated disks, as assessed by immunofluorescence. It is concluded that adult cardiomyocytes express functionally coupled high- affinity bFGF receptors and that they are capable of a biologic response to bFGF in vivo.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume268
Issue number5 37-5
Publication statusPublished - 1 Jan 1995
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Cardiac Myocytes
Fibroblast Growth Factor Receptors
Muscle Cells
Myocardium
Receptor, Fibroblast Growth Factor, Type 1
Messenger RNA
Mitogen-Activated Protein Kinases
Protein-Tyrosine Kinases
Fluorescent Antibody Technique
Tyrosine
Heparin
Perfusion
Phosphorylation

Keywords

  • basic fibroblast growth factor receptor 1 variants
  • signal transduction

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Liu, L., Pasumarthi, K. B. S., Padua, R. R., Massaeli, H., Fandrich, R. R., Pierce, G. N., ... Kardami, E. (1995). Adult cardiomyocytes express functional high-affinity receptors for basic fibroblast growth factor. American Journal of Physiology - Heart and Circulatory Physiology, 268(5 37-5).

Adult cardiomyocytes express functional high-affinity receptors for basic fibroblast growth factor. / Liu, L.; Pasumarthi, K. B.S.; Padua, R. R.; Massaeli, Hamid; Fandrich, R. R.; Pierce, G. N.; Cattini, P. A.; Kardami, E.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 268, No. 5 37-5, 01.01.1995.

Research output: Contribution to journalArticle

Liu, L, Pasumarthi, KBS, Padua, RR, Massaeli, H, Fandrich, RR, Pierce, GN, Cattini, PA & Kardami, E 1995, 'Adult cardiomyocytes express functional high-affinity receptors for basic fibroblast growth factor', American Journal of Physiology - Heart and Circulatory Physiology, vol. 268, no. 5 37-5.
Liu, L. ; Pasumarthi, K. B.S. ; Padua, R. R. ; Massaeli, Hamid ; Fandrich, R. R. ; Pierce, G. N. ; Cattini, P. A. ; Kardami, E. / Adult cardiomyocytes express functional high-affinity receptors for basic fibroblast growth factor. In: American Journal of Physiology - Heart and Circulatory Physiology. 1995 ; Vol. 268, No. 5 37-5.
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