Adsorption of model charged proteins on charged surfaces with grafted polymers

Marcelo Carignano, Igal Szleifer

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The adsorption of model charged proteins on charged surfaces with and without grafted polymers is studied using a molecular approach. The ability of the polymer layer to reduce the amount of proteins adsorbed on top of the surface (primary adsorption) and at the same time to increase the adsorption of the proteins on top of the polymer layer (secondary adsorption) is presented. It is found that charging the free ends of the chains can result in an efficient way to enhance adsorption at the tip of the brush. Increasing the surface coverage of the polymers with charged free ends enhances the amount of proteins adsorbed at the tip of the polymer layer, while at the same time strongly reduces the number of proteins adsorbed directly onto the surface. The interplay between the attractive van der Waals protein-surface interactions, the steric polymer-protein interactions and the effect of the electrostatic interactions in determining the final adsorption is discussed. The manipulation of solution conditions to tune the amount of secondary adsorption is presented.

Original languageEnglish
Pages (from-to)2993-3003
Number of pages11
JournalMolecular Physics
Volume100
Issue number18
DOIs
Publication statusPublished - 20 Sep 2002
Externally publishedYes

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Adsorption
Polymers
proteins
adsorption
polymers
Proteins
brushes
Brushes
Coulomb interactions
Static Electricity
surface reactions
charging
manipulators
Membrane Proteins
interactions
electrostatics

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Adsorption of model charged proteins on charged surfaces with grafted polymers. / Carignano, Marcelo; Szleifer, Igal.

In: Molecular Physics, Vol. 100, No. 18, 20.09.2002, p. 2993-3003.

Research output: Contribution to journalArticle

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