Adenovirus-mediated transfer of a recombinant α1-antitrypsin gene to the lung epithelium in vivo

Melissa A. Rosenfeld, Wolfgang Siegfried, Kunihiko Yoshimura, Koichi Yoneyama, Masashi Fukayama, Larue E. Stier, Paavo K. Pääkkö, Pascale Gilardi, Leslie D. Stratford-Perricaudet, Michel Perricaudet, Sophie Jallat, Andrea Pavirani, Jean Pierre Lecocq, Ronald Crystal

Research output: Contribution to journalArticle

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Abstract

The respiratory epithelium is a potential site for somatic gene therapy for the common hereditary disorders α1-antitrypsin (α1AT) deficiency and cystic fibrosis. A replication-deficient adenoviral vector (Ad-α1AT) containing an adenovirus major late promoter and a recombinant human α1AT gene was used to infect epithelial cells of the cotton rat respiratory tract in vitro and in vivo. Freshly isolated tracheobronchial epithelial cells infected with Ad-α1AT contained human α1AT messenger RNA transcripts and synthesized and secreted human α1AT. After in vivo intratracheal administration of Ad-α1AT to these rats, human α1AT messenger RNA was observed in the respiratory epithelium, human α1AT was synthesized and secreted by lung tissue, and human α1AT was detected in the epithelial lining fluid for at least 1 week.

Original languageEnglish
Pages (from-to)431-434
Number of pages4
JournalScience
Volume252
Issue number5004
Publication statusPublished - 1 Jan 1991
Externally publishedYes

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Adenoviridae
Epithelium
Lung
Genes
Respiratory Mucosa
Epithelial Cells
Sigmodontinae
Messenger RNA
Cystic Fibrosis
Genetic Therapy
Respiratory System

ASJC Scopus subject areas

  • General

Cite this

Rosenfeld, M. A., Siegfried, W., Yoshimura, K., Yoneyama, K., Fukayama, M., Stier, L. E., ... Crystal, R. (1991). Adenovirus-mediated transfer of a recombinant α1-antitrypsin gene to the lung epithelium in vivo. Science, 252(5004), 431-434.

Adenovirus-mediated transfer of a recombinant α1-antitrypsin gene to the lung epithelium in vivo. / Rosenfeld, Melissa A.; Siegfried, Wolfgang; Yoshimura, Kunihiko; Yoneyama, Koichi; Fukayama, Masashi; Stier, Larue E.; Pääkkö, Paavo K.; Gilardi, Pascale; Stratford-Perricaudet, Leslie D.; Perricaudet, Michel; Jallat, Sophie; Pavirani, Andrea; Lecocq, Jean Pierre; Crystal, Ronald.

In: Science, Vol. 252, No. 5004, 01.01.1991, p. 431-434.

Research output: Contribution to journalArticle

Rosenfeld, MA, Siegfried, W, Yoshimura, K, Yoneyama, K, Fukayama, M, Stier, LE, Pääkkö, PK, Gilardi, P, Stratford-Perricaudet, LD, Perricaudet, M, Jallat, S, Pavirani, A, Lecocq, JP & Crystal, R 1991, 'Adenovirus-mediated transfer of a recombinant α1-antitrypsin gene to the lung epithelium in vivo', Science, vol. 252, no. 5004, pp. 431-434.
Rosenfeld MA, Siegfried W, Yoshimura K, Yoneyama K, Fukayama M, Stier LE et al. Adenovirus-mediated transfer of a recombinant α1-antitrypsin gene to the lung epithelium in vivo. Science. 1991 Jan 1;252(5004):431-434.
Rosenfeld, Melissa A. ; Siegfried, Wolfgang ; Yoshimura, Kunihiko ; Yoneyama, Koichi ; Fukayama, Masashi ; Stier, Larue E. ; Pääkkö, Paavo K. ; Gilardi, Pascale ; Stratford-Perricaudet, Leslie D. ; Perricaudet, Michel ; Jallat, Sophie ; Pavirani, Andrea ; Lecocq, Jean Pierre ; Crystal, Ronald. / Adenovirus-mediated transfer of a recombinant α1-antitrypsin gene to the lung epithelium in vivo. In: Science. 1991 ; Vol. 252, No. 5004. pp. 431-434.
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