Adenovirus gene transfer vectors inhibit growth of lymphatic tumor metastases independent of a therapeutic transgene

R. J. Korst, M. Ailawadi, J. M. Lee, S. Lee, R. Yamada, A. Mahtabifard, Ronald Crystal

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Adenovirus (Ad) gene transfer vectors traffic to regional lymph nodes (RLNs) after footpad injections in mice, resulting in localized production of interferon γ (IFN-γ). With this background, we evaluated the hypothesis that Ad vector administration may inhibit RLN tumor metastasis independent of the transgene in the expression cassette. Tumors of MM48, a cell line with a propensity toward lymphogenous metastasis, were established in the footpads of syngeneic C3H mice, and E1-E3- Ad vectors encoding no transgene (AdNull) or encoding an irrelevant transgene (AdCD; Escherichia coli cytosine deaminase with no 5-fluorocytosine administration) were administered (1010 particles) in a peritumoral location. Both vectors suppressed the growth of tumor in the regional (popliteal) lymph node. This effect was localized to the regional, but not distant, lymph nodes (p < 0.05). Heat inactivation of the vector or decreasing the dose of the vector to 109 particles did not suppress RLN growth of the tumor when compared with 1010 particles of active AdNull (p < 0.05 and p < 0.01, respectively). The ability of an E1-E4- vector expressing β-galactosidase (AdRSVβgal.11) to suppress RLN tumor growth showed that the E4 region of the Ad vector was not responsible for the effect. Blocking either IFN-γ or natural killer (NK) cells with systemic antibody treatment in immunocompetent mice allowed rapid growth of RLN metastases despite Ad vector administration, and Ad vector injection into the footpads of tumor-free mice induced the accumulation of NK cells in the RLN. These data demonstrate that, in a metastatic murine tumor model, a low dose (1010 particles) of replication-deficient Ad vectors inhibits RLN metastases independent of a therapeutic transgene, an effect that is mediated, at least in part, by IFN-γ and NK cells.

Original languageEnglish
Pages (from-to)1639-1649
Number of pages11
JournalHuman Gene Therapy
Volume12
Issue number13
DOIs
Publication statusPublished - 10 Oct 2001
Externally publishedYes

Fingerprint

Lymphatic Metastasis
Transgenes
Adenoviridae
Lymph Nodes
Growth
Genes
Neoplasms
Natural Killer Cells
Interferons
Neoplasm Metastasis
Therapeutics
Cytosine Deaminase
Galactosidases
Flucytosine
Injections
Inbred C3H Mouse
Therapeutic Uses
Hot Temperature
Escherichia coli
Cell Line

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Adenovirus gene transfer vectors inhibit growth of lymphatic tumor metastases independent of a therapeutic transgene. / Korst, R. J.; Ailawadi, M.; Lee, J. M.; Lee, S.; Yamada, R.; Mahtabifard, A.; Crystal, Ronald.

In: Human Gene Therapy, Vol. 12, No. 13, 10.10.2001, p. 1639-1649.

Research output: Contribution to journalArticle

Korst, R. J. ; Ailawadi, M. ; Lee, J. M. ; Lee, S. ; Yamada, R. ; Mahtabifard, A. ; Crystal, Ronald. / Adenovirus gene transfer vectors inhibit growth of lymphatic tumor metastases independent of a therapeutic transgene. In: Human Gene Therapy. 2001 ; Vol. 12, No. 13. pp. 1639-1649.
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