Addition of thrombin reduces the recovery of extracellular vesicles from blood plasma

Anush Arakelyan, Wendy Fitzgerald, Murad Vagida, Elena Vasilieva, Leonid Margolis, Jean-Charles B. Grivel

Research output: Contribution to journalArticle

Abstract

Extracellular vesicles (EVs) are widely studied as a system of intercellular communication, as markers of various diseases, as well as a vehicle for delivery of various bioactive molecules to various cells. Investigation of EVs’ structure and function requires their isolation and precise quantification. However, in the current literature, there are significant discrepancies in the estimated numbers of EVs in different body fluids. In part, this discrepancy is due to the difference in EVs isolation protocols used by different investigators. A common protocol that includes ExoQuick is often used to isolate EVs from body fluids and culture medium. Here, we show that in the case of isolation of EVs from blood, thrombin should be omitted from the protocol as clots formed due to the thrombin-triggered coagulation may entrap many EVs thus leading to the underestimation of their numbers.

Original languageEnglish
JournalJournal of Circulating Biomarkers
Volume5
DOIs
Publication statusPublished - 4 Oct 2016

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Keywords

  • Extracellular vesicles
  • nanoparticles
  • quantification
  • thrombin

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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