Acute systemic exposure to silver-based nanoparticles induces hepatotoxicity and NLRP3-dependent inflammation

Khalil B. Ramadi, Yassir A. Mohamed, Ashraf Al-Sbiei, Saeeda Almarzooqi, Ghada Bashir, Aisha Al Dhanhani, Dhanya Sarawathiamma, Shahnaz Qadri, Javed Yasin, Abderrahim Nemmar, Maria J. Fernandez-Cabezudo, Yousef Haik, Basel K. Al-Ramadi

Research output: Contribution to journalArticle

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Abstract

Nanoparticles (NPs) are increasingly being commercialized for use in biomedicine. NP toxicity following acute or chronic exposure has been described, but mechanistic insight into this process remains incomplete. Recent evidence from in vitro studies suggested a role for NLRP3 in NP cytotoxicity. In this study, we investigated the effect of systemic administration of composite inorganic NP, consisting of Ag:Cu:B (dose range 1–20 mg/kg), on the early acute (4–24 h post-exposure) and late phase response (96 h post-exposure) in normal and NLRP3-deficient mice. Our findings indicate that systemic exposure (≥2 mg/kg) was associated with acute liver injury due to preferential accumulation of NP in this organ and resulted in elevated AST, ALT and LDH levels. Moreover, within 24 h of NP administration, there was a dose-dependent increase in intraperitoneal neutrophil recruitment and upregulation in gene expression of several proinflammatory mediators, including TNF-α, IL-1β and S100A9. Histological analysis of liver tissue revealed evidence of dose-dependent hepatocyte necrosis, increase in sinusoidal Kupffer cells, lobular granulomas and foci of abscess formation which were most pronounced at 24 h following NP administration. NP deposition in the liver led to a significant upregulation in gene expression of S100A9, an endogenous danger signal recognition molecule of phagocytes, IL-1β and IL-6. The extent of proinflammatory cytokine activation and hepatotoxicity was significantly attenuated in mice deficient in the NLRP3 inflammasome, demonstrating the critical role of this innate immune system recognition receptor in the response to NP.

Original languageEnglish
Pages (from-to)1061-1074
Number of pages14
JournalNanotoxicology
Volume10
Issue number8
DOIs
Publication statusPublished - 13 Sep 2016
Externally publishedYes

Fingerprint

Silver
Nanoparticles
Inflammation
Liver
Interleukin-1
Gene expression
Up-Regulation
Inflammasomes
Gene Expression
Kupffer Cells
Neutrophil Infiltration
Immune system
Cytotoxicity
Phagocytes
Granuloma
Abscess
Toxicity
Hepatocytes
Immune System
Interleukin-6

Keywords

  • Acute nanotoxicity
  • hepatotoxicity
  • IL-1β
  • NLRP3
  • S100A9
  • silver nanoparticles

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

Cite this

Ramadi, K. B., Mohamed, Y. A., Al-Sbiei, A., Almarzooqi, S., Bashir, G., Al Dhanhani, A., ... Al-Ramadi, B. K. (2016). Acute systemic exposure to silver-based nanoparticles induces hepatotoxicity and NLRP3-dependent inflammation. Nanotoxicology, 10(8), 1061-1074. https://doi.org/10.3109/17435390.2016.1163743

Acute systemic exposure to silver-based nanoparticles induces hepatotoxicity and NLRP3-dependent inflammation. / Ramadi, Khalil B.; Mohamed, Yassir A.; Al-Sbiei, Ashraf; Almarzooqi, Saeeda; Bashir, Ghada; Al Dhanhani, Aisha; Sarawathiamma, Dhanya; Qadri, Shahnaz; Yasin, Javed; Nemmar, Abderrahim; Fernandez-Cabezudo, Maria J.; Haik, Yousef; Al-Ramadi, Basel K.

In: Nanotoxicology, Vol. 10, No. 8, 13.09.2016, p. 1061-1074.

Research output: Contribution to journalArticle

Ramadi, KB, Mohamed, YA, Al-Sbiei, A, Almarzooqi, S, Bashir, G, Al Dhanhani, A, Sarawathiamma, D, Qadri, S, Yasin, J, Nemmar, A, Fernandez-Cabezudo, MJ, Haik, Y & Al-Ramadi, BK 2016, 'Acute systemic exposure to silver-based nanoparticles induces hepatotoxicity and NLRP3-dependent inflammation', Nanotoxicology, vol. 10, no. 8, pp. 1061-1074. https://doi.org/10.3109/17435390.2016.1163743
Ramadi KB, Mohamed YA, Al-Sbiei A, Almarzooqi S, Bashir G, Al Dhanhani A et al. Acute systemic exposure to silver-based nanoparticles induces hepatotoxicity and NLRP3-dependent inflammation. Nanotoxicology. 2016 Sep 13;10(8):1061-1074. https://doi.org/10.3109/17435390.2016.1163743
Ramadi, Khalil B. ; Mohamed, Yassir A. ; Al-Sbiei, Ashraf ; Almarzooqi, Saeeda ; Bashir, Ghada ; Al Dhanhani, Aisha ; Sarawathiamma, Dhanya ; Qadri, Shahnaz ; Yasin, Javed ; Nemmar, Abderrahim ; Fernandez-Cabezudo, Maria J. ; Haik, Yousef ; Al-Ramadi, Basel K. / Acute systemic exposure to silver-based nanoparticles induces hepatotoxicity and NLRP3-dependent inflammation. In: Nanotoxicology. 2016 ; Vol. 10, No. 8. pp. 1061-1074.
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