Activation of human T cells with the physiological regulator of protein kinase C

A. Subramaniam, P. Sehajpal, V. K. Murthi, K. H. Stenzel, Manikkam Suthanthiran

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We investigated whether, sn-1,2-dioctanoylglycerol (diC8) activates highly purified human T cells. diC8's signaling activity was also compared with that of 12-O-tetradecanoylphorbol-13-acetate (TPA). diC8 and ionomycin were synergistic in promoting T-cell proliferation. The proliferative response was dependent upon an operational interleukin-2 (IL-2) system and exhibited a high degree of specificity; sn-1,2-diC8 was twice as active as racemic-1,2-diC8, and diC8 and TPA were not synergistic, diC8's signaling activity differed from that of TPA. diC8, unlike TPA, failed to elicit IL-2 receptors or proliferation, independently of ionomycin. diC8 also failed to promote the proliferation of T cells signaled with anti-CD3 or -CD2 monoclonal antibodies. Two different inhibitors of PKC, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine or staurosporine, inhibited T-cell proliferation induced with diC8 and ionomycin, but not with TPA and ionomycin. These observations, in addition to demonstrating the differential activity of diC8 and TPA, document a signaling role for diacylglycerol in the activation of normal T cells.

Original languageEnglish
Pages (from-to)439-449
Number of pages11
JournalCellular Immunology
Volume116
Issue number2
DOIs
Publication statusPublished - 15 Oct 1988
Externally publishedYes

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Protein Kinase C
T-Lymphocytes
Tetradecanoylphorbol Acetate
Ionomycin
1,2-dioctanoylglycerol
Cell Proliferation
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Staurosporine
Interleukin-2 Receptors
Diglycerides
Interleukin-2
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Activation of human T cells with the physiological regulator of protein kinase C. / Subramaniam, A.; Sehajpal, P.; Murthi, V. K.; Stenzel, K. H.; Suthanthiran, Manikkam.

In: Cellular Immunology, Vol. 116, No. 2, 15.10.1988, p. 439-449.

Research output: Contribution to journalArticle

Subramaniam, A. ; Sehajpal, P. ; Murthi, V. K. ; Stenzel, K. H. ; Suthanthiran, Manikkam. / Activation of human T cells with the physiological regulator of protein kinase C. In: Cellular Immunology. 1988 ; Vol. 116, No. 2. pp. 439-449.
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