Activation of chloride secretion via proteinase-activated receptor 2 in a human eccrine sweat gland cell line - NCL-SG3

Douglas Bovell, Radmila Santic, Barbara Kofler, Anton Hermann, Darius Wilson, Alistair Corbett, Roland Lang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Proteinase-activated receptor 2 (PAR-2) has been shown to elicit secretion in a variety of secretory epithelial cells by the transepithelial movement of chloride ions across the apical membrane. However, it is not known whether these receptors are present and/or functional in the secretory epithelial cells of the human eccrine sweat gland. To investigate this possibility mRNA analysis, Ca2+ microspectrofluorimetry and the short circuit current (Isc) technique were used to quantify electrolyte transport in a cell line (NCL-SG3) derived from human eccrine sweat gland secretory epithelia. The results provided molecular and functional evidence of the presence of PAR-2 receptors in the NCL-SG3 cells and show that these receptors can activate transepithelial Cl- secretion possibly via Ca2+-activated Cl- channels.

Original languageEnglish
Pages (from-to)505-511
Number of pages7
JournalExperimental Dermatology
Volume17
Issue number6
DOIs
Publication statusPublished - Jun 2008
Externally publishedYes

Fingerprint

PAR-2 Receptor
Eccrine Glands
Sweat Glands
Chlorides
Epithelial Cells
Chemical activation
Cells
Cell Line
Short circuit currents
Electrolytes
Cell Movement
Epithelium
Ions
Membranes
Messenger RNA

Keywords

  • Eccrine sweat gland cells
  • Ion transport
  • NCL-SG3 cells
  • PAR-2
  • Proteinase-activated receptors

ASJC Scopus subject areas

  • Dermatology

Cite this

Activation of chloride secretion via proteinase-activated receptor 2 in a human eccrine sweat gland cell line - NCL-SG3. / Bovell, Douglas; Santic, Radmila; Kofler, Barbara; Hermann, Anton; Wilson, Darius; Corbett, Alistair; Lang, Roland.

In: Experimental Dermatology, Vol. 17, No. 6, 06.2008, p. 505-511.

Research output: Contribution to journalArticle

Bovell, Douglas ; Santic, Radmila ; Kofler, Barbara ; Hermann, Anton ; Wilson, Darius ; Corbett, Alistair ; Lang, Roland. / Activation of chloride secretion via proteinase-activated receptor 2 in a human eccrine sweat gland cell line - NCL-SG3. In: Experimental Dermatology. 2008 ; Vol. 17, No. 6. pp. 505-511.
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