Acid-induced structural changes in human rhinovirus 14: Possible role in uncoating

Vincent L. Giranda, Beverly A. Heinz, Marcos A. Oliveira, Iwona Minor, Kyung Hyun Kim, Prasanna Kolatkar, Michael G. Rossmann, Roland R. Rueckert

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Abstract

X-ray diffraction data were collected from human rhinovirus 14 crystals a few minutes after exposure to acid vapor and prior to excessive crystalline disorder. Conformational changes occurred (i) in the GH loop of viral protein (VP) 1, (ii) at the ion binding site on the outer surface of the pentamer center, and (iii) in VP3 and VP4 on the virion's interior in the vicinity of the fivefold axis. Amino acid substitutions in mutants resistant to low pH, or to drugs that inhibit uncoating, were concentrated in the vicinity of the GH loop. It is proposed that the acid-induced changes reflect processes that trigger uncoating.

Original languageEnglish
Pages (from-to)10213-10217
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number21
Publication statusPublished - 1 Dec 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Giranda, V. L., Heinz, B. A., Oliveira, M. A., Minor, I., Kim, K. H., Kolatkar, P., Rossmann, M. G., & Rueckert, R. R. (1992). Acid-induced structural changes in human rhinovirus 14: Possible role in uncoating. Proceedings of the National Academy of Sciences of the United States of America, 89(21), 10213-10217.