Accessibility of the globular domain of histones H1 and H5 to antibodies upon folding of chromatin

Valya R. RUSSANOVA, Stephan I. DIMITROV, Vladimir L. MAKAROV, Iliya G. PASHEV

Research output: Contribution to journalArticle

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Abstract

Antibodies to the globular domain of histones H1 and H5 were purified by affinity chromatography and used to study the accessibility of this region of H1 and H5 in folded and unfolded rat liver and hen erythrocyte chromatin respectively. The different conformations of the chromatin filament were induced by varying the ionic strength from 1 mM to 80 mM NaCl and maintained by fixation with glutaraldehyde. Treatment with glutaraldehyde at a given salt concentration affected neither the orientation of nucleosomes relative to the fiber axis nor the compactness of chromatin. Solid‐phase immunoassay and inhibition experiments showed no binding of the antibody against the globular domain of H1 to chromatin at the entire range of salt concentrations, while the antibody to the whole H1 molecule reacted with chromatin at low salt. On the other hand, the antibody to the globular region of H5 reacted with hen erythrocyte chromatin independently of the extent of chromatin condensation. These results indicate that the antigenic determinants of the globular domain of H5 are accessible to the antibody both in folded and unfolded chromatin, while those of the same region of H1 are masked, probably by interaction with DNA or proteins.

Original languageEnglish
Pages (from-to)321-326
Number of pages6
JournalEuropean Journal of Biochemistry
Volume167
Issue number2
DOIs
Publication statusPublished - 1987
Externally publishedYes

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Histones
Chromatin
Antibodies
Salts
Glutaral
Erythrocytes
Affinity chromatography
Nucleosomes
Ionic strength
Affinity Chromatography
Immunoassay
Liver
Osmolar Concentration
Conformations
Rats
Epitopes
Condensation
Molecules
Fibers
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Accessibility of the globular domain of histones H1 and H5 to antibodies upon folding of chromatin. / RUSSANOVA, Valya R.; DIMITROV, Stephan I.; MAKAROV, Vladimir L.; PASHEV, Iliya G.

In: European Journal of Biochemistry, Vol. 167, No. 2, 1987, p. 321-326.

Research output: Contribution to journalArticle

RUSSANOVA, Valya R. ; DIMITROV, Stephan I. ; MAKAROV, Vladimir L. ; PASHEV, Iliya G. / Accessibility of the globular domain of histones H1 and H5 to antibodies upon folding of chromatin. In: European Journal of Biochemistry. 1987 ; Vol. 167, No. 2. pp. 321-326.
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