A universal nanoparticle cell secretion capture assay

Wendy Fitzgerald, Jean-Charles B. Grivel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Secreted proteins play an important role in intercellular interactions, especially between cells of the immune system. Currently, there is no universal assay that allows a simple noninvasive identification and isolation of cells based on their secretion of various products. We have developed such a method. Our method is based on the targeting, to the cell surface, of heterofunctional nanoparticles coupled to a cell surface-specific antibody and to a secreted protein-specific antibody, which captures the secreted protein on the surface of the producing cell. Importantly, this method does not compromise cellviability and is compatible with further culture and expansion of the secreting cells. Published 2012 Wiley Periodicals, Inc.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalCytometry Part A
Volume83 A
Issue number2
DOIs
Publication statusPublished - Feb 2013
Externally publishedYes

Fingerprint

Nanoparticles
Antibodies
Cell Separation
Immune System
Membrane Proteins
Proteins

Keywords

  • Capture
  • Cytokine
  • Nanoparticle
  • Secretion

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

Cite this

A universal nanoparticle cell secretion capture assay. / Fitzgerald, Wendy; Grivel, Jean-Charles B.

In: Cytometry Part A, Vol. 83 A, No. 2, 02.2013, p. 205-211.

Research output: Contribution to journalArticle

Fitzgerald, Wendy ; Grivel, Jean-Charles B. / A universal nanoparticle cell secretion capture assay. In: Cytometry Part A. 2013 ; Vol. 83 A, No. 2. pp. 205-211.
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