A Toolbox for Tuberculosis (TB) Diagnosis: An Indian Multicentric Study (2006-2008). Evaluation of QuantiFERON-TB Gold in Tube for TB Diagnosis

Philippe H. Lagrange, Satheesh K. Thangaraj, Rajeshwar Dayal, Alaka Deshpande, Nirmal K. Ganguly, Enrico Girardi, Beenu Joshi, Kiran Katoch, Vishwa M. Katoch, Manoj Kumar, Vemu Lakshmi, Marc Leportier, Christophe Longuet, Subbalaxmi V S Malladi, Deepali Mukerjee, Deepthi Nair, Alamelu Raja, Balambal Raman, Camilla Rodrigues, Pratibha SharmaAmit Singh, Sarman Singh, Archana Sodha, Basirudeen S. Kabeer, Guy Vernet, Delia Goletti

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Abstract

Background:The aim of this multicentric prospective study in India was to assess the performance of the QuantiFERON TB-Gold in tube (QFT-GIT), Tuberculin Skin Test (TST) and microbiological results as additional tools for diagnosing active tuberculosis (TB) and latent infection (LTBI) according to Human Immunodeficiency Virus (HIV) status.Methods:Individuals with and without active TB and HIV infection were enrolled between 2006-2008. QFT-GIT and TST results were analyzed per se and in combination with microbiological data.Results:Among the 276 individuals (96 active pulmonary TB and 180 no active TB) tested by QFT-GIT, 18 indeterminate results (6.5%) were found, more significantly numerous in the HIV-infected (15/92; 16.3%) than the HIV-uninfected (3/184; 1.6%)(p<0.0001). QFT-GIT sensitivity for active TB was 82.3% and 92.9% respectively after including or excluding indeterminate results. Clinical sensitivity was significantly lower in the HIV-infected (68.4%) than the HIV-uninfected (91.4%) patients (p = 0.0059). LTBI was detected in 49.3% of subjects without active TB but varied according to TB exposure. When the TST and QFT-GIT were concomitantly performed, the respective sensitivity for active TB diagnosis was 95.0% and 85.0% in the HIV-uninfected (p = 0.60), and 66.7% and 51.5% in the HIV-infected patients (p = 0.32). QFT-GIT and TST respective specificity for active TB in the HIV-uninfected was 25.0% and 57.1% (p = 0.028), and 64.8% and 83.3% in the HIV-infected (p = 0.047). In those with active TB, QFT-GIT results were not associated with microbiological parameters (smear grade, liquid culture status, time-to-positivity of culture) or clinical suspicion of active TB score (provided by the clinicians at enrollment). Combining microbiological tests with both immunological tests significantly increased sensitivity for active TB diagnosis (p = 0.0002), especially in the HIV-infected individuals (p = 0.0016).Conclusion:QFT-GIT and TST have similar diagnostic value for active TB diagnosis. In HIV-infected patients, combining microbiological tests with both immunological tests significantly increases the sensitivity for active TB diagnosis.

Original languageEnglish
Article numbere73579
JournalPLoS One
Volume8
Issue number9
DOIs
Publication statusPublished - 6 Sep 2013
Externally publishedYes

Fingerprint

Viruses
tuberculosis
Gold
gold
Human immunodeficiency virus
Tuberculosis
HIV
Tuberculin Test
Tuberculin
tuberculin
Skin Tests
skin tests
Skin
Immunologic Tests
immunologic techniques
Latent Tuberculosis
Virus Diseases
Pulmonary Tuberculosis
HIV infections
disease diagnosis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lagrange, P. H., Thangaraj, S. K., Dayal, R., Deshpande, A., Ganguly, N. K., Girardi, E., ... Goletti, D. (2013). A Toolbox for Tuberculosis (TB) Diagnosis: An Indian Multicentric Study (2006-2008). Evaluation of QuantiFERON-TB Gold in Tube for TB Diagnosis. PLoS One, 8(9), [e73579]. https://doi.org/10.1371/journal.pone.0073579

A Toolbox for Tuberculosis (TB) Diagnosis : An Indian Multicentric Study (2006-2008). Evaluation of QuantiFERON-TB Gold in Tube for TB Diagnosis. / Lagrange, Philippe H.; Thangaraj, Satheesh K.; Dayal, Rajeshwar; Deshpande, Alaka; Ganguly, Nirmal K.; Girardi, Enrico; Joshi, Beenu; Katoch, Kiran; Katoch, Vishwa M.; Kumar, Manoj; Lakshmi, Vemu; Leportier, Marc; Longuet, Christophe; Malladi, Subbalaxmi V S; Mukerjee, Deepali; Nair, Deepthi; Raja, Alamelu; Raman, Balambal; Rodrigues, Camilla; Sharma, Pratibha; Singh, Amit; Singh, Sarman; Sodha, Archana; Kabeer, Basirudeen S.; Vernet, Guy; Goletti, Delia.

In: PLoS One, Vol. 8, No. 9, e73579, 06.09.2013.

Research output: Contribution to journalArticle

Lagrange, PH, Thangaraj, SK, Dayal, R, Deshpande, A, Ganguly, NK, Girardi, E, Joshi, B, Katoch, K, Katoch, VM, Kumar, M, Lakshmi, V, Leportier, M, Longuet, C, Malladi, SVS, Mukerjee, D, Nair, D, Raja, A, Raman, B, Rodrigues, C, Sharma, P, Singh, A, Singh, S, Sodha, A, Kabeer, BS, Vernet, G & Goletti, D 2013, 'A Toolbox for Tuberculosis (TB) Diagnosis: An Indian Multicentric Study (2006-2008). Evaluation of QuantiFERON-TB Gold in Tube for TB Diagnosis', PLoS One, vol. 8, no. 9, e73579. https://doi.org/10.1371/journal.pone.0073579
Lagrange, Philippe H. ; Thangaraj, Satheesh K. ; Dayal, Rajeshwar ; Deshpande, Alaka ; Ganguly, Nirmal K. ; Girardi, Enrico ; Joshi, Beenu ; Katoch, Kiran ; Katoch, Vishwa M. ; Kumar, Manoj ; Lakshmi, Vemu ; Leportier, Marc ; Longuet, Christophe ; Malladi, Subbalaxmi V S ; Mukerjee, Deepali ; Nair, Deepthi ; Raja, Alamelu ; Raman, Balambal ; Rodrigues, Camilla ; Sharma, Pratibha ; Singh, Amit ; Singh, Sarman ; Sodha, Archana ; Kabeer, Basirudeen S. ; Vernet, Guy ; Goletti, Delia. / A Toolbox for Tuberculosis (TB) Diagnosis : An Indian Multicentric Study (2006-2008). Evaluation of QuantiFERON-TB Gold in Tube for TB Diagnosis. In: PLoS One. 2013 ; Vol. 8, No. 9.
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title = "A Toolbox for Tuberculosis (TB) Diagnosis: An Indian Multicentric Study (2006-2008). Evaluation of QuantiFERON-TB Gold in Tube for TB Diagnosis",
abstract = "Background:The aim of this multicentric prospective study in India was to assess the performance of the QuantiFERON TB-Gold in tube (QFT-GIT), Tuberculin Skin Test (TST) and microbiological results as additional tools for diagnosing active tuberculosis (TB) and latent infection (LTBI) according to Human Immunodeficiency Virus (HIV) status.Methods:Individuals with and without active TB and HIV infection were enrolled between 2006-2008. QFT-GIT and TST results were analyzed per se and in combination with microbiological data.Results:Among the 276 individuals (96 active pulmonary TB and 180 no active TB) tested by QFT-GIT, 18 indeterminate results (6.5{\%}) were found, more significantly numerous in the HIV-infected (15/92; 16.3{\%}) than the HIV-uninfected (3/184; 1.6{\%})(p<0.0001). QFT-GIT sensitivity for active TB was 82.3{\%} and 92.9{\%} respectively after including or excluding indeterminate results. Clinical sensitivity was significantly lower in the HIV-infected (68.4{\%}) than the HIV-uninfected (91.4{\%}) patients (p = 0.0059). LTBI was detected in 49.3{\%} of subjects without active TB but varied according to TB exposure. When the TST and QFT-GIT were concomitantly performed, the respective sensitivity for active TB diagnosis was 95.0{\%} and 85.0{\%} in the HIV-uninfected (p = 0.60), and 66.7{\%} and 51.5{\%} in the HIV-infected patients (p = 0.32). QFT-GIT and TST respective specificity for active TB in the HIV-uninfected was 25.0{\%} and 57.1{\%} (p = 0.028), and 64.8{\%} and 83.3{\%} in the HIV-infected (p = 0.047). In those with active TB, QFT-GIT results were not associated with microbiological parameters (smear grade, liquid culture status, time-to-positivity of culture) or clinical suspicion of active TB score (provided by the clinicians at enrollment). Combining microbiological tests with both immunological tests significantly increased sensitivity for active TB diagnosis (p = 0.0002), especially in the HIV-infected individuals (p = 0.0016).Conclusion:QFT-GIT and TST have similar diagnostic value for active TB diagnosis. In HIV-infected patients, combining microbiological tests with both immunological tests significantly increases the sensitivity for active TB diagnosis.",
author = "Lagrange, {Philippe H.} and Thangaraj, {Satheesh K.} and Rajeshwar Dayal and Alaka Deshpande and Ganguly, {Nirmal K.} and Enrico Girardi and Beenu Joshi and Kiran Katoch and Katoch, {Vishwa M.} and Manoj Kumar and Vemu Lakshmi and Marc Leportier and Christophe Longuet and Malladi, {Subbalaxmi V S} and Deepali Mukerjee and Deepthi Nair and Alamelu Raja and Balambal Raman and Camilla Rodrigues and Pratibha Sharma and Amit Singh and Sarman Singh and Archana Sodha and Kabeer, {Basirudeen S.} and Guy Vernet and Delia Goletti",
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TY - JOUR

T1 - A Toolbox for Tuberculosis (TB) Diagnosis

T2 - An Indian Multicentric Study (2006-2008). Evaluation of QuantiFERON-TB Gold in Tube for TB Diagnosis

AU - Lagrange, Philippe H.

AU - Thangaraj, Satheesh K.

AU - Dayal, Rajeshwar

AU - Deshpande, Alaka

AU - Ganguly, Nirmal K.

AU - Girardi, Enrico

AU - Joshi, Beenu

AU - Katoch, Kiran

AU - Katoch, Vishwa M.

AU - Kumar, Manoj

AU - Lakshmi, Vemu

AU - Leportier, Marc

AU - Longuet, Christophe

AU - Malladi, Subbalaxmi V S

AU - Mukerjee, Deepali

AU - Nair, Deepthi

AU - Raja, Alamelu

AU - Raman, Balambal

AU - Rodrigues, Camilla

AU - Sharma, Pratibha

AU - Singh, Amit

AU - Singh, Sarman

AU - Sodha, Archana

AU - Kabeer, Basirudeen S.

AU - Vernet, Guy

AU - Goletti, Delia

PY - 2013/9/6

Y1 - 2013/9/6

N2 - Background:The aim of this multicentric prospective study in India was to assess the performance of the QuantiFERON TB-Gold in tube (QFT-GIT), Tuberculin Skin Test (TST) and microbiological results as additional tools for diagnosing active tuberculosis (TB) and latent infection (LTBI) according to Human Immunodeficiency Virus (HIV) status.Methods:Individuals with and without active TB and HIV infection were enrolled between 2006-2008. QFT-GIT and TST results were analyzed per se and in combination with microbiological data.Results:Among the 276 individuals (96 active pulmonary TB and 180 no active TB) tested by QFT-GIT, 18 indeterminate results (6.5%) were found, more significantly numerous in the HIV-infected (15/92; 16.3%) than the HIV-uninfected (3/184; 1.6%)(p<0.0001). QFT-GIT sensitivity for active TB was 82.3% and 92.9% respectively after including or excluding indeterminate results. Clinical sensitivity was significantly lower in the HIV-infected (68.4%) than the HIV-uninfected (91.4%) patients (p = 0.0059). LTBI was detected in 49.3% of subjects without active TB but varied according to TB exposure. When the TST and QFT-GIT were concomitantly performed, the respective sensitivity for active TB diagnosis was 95.0% and 85.0% in the HIV-uninfected (p = 0.60), and 66.7% and 51.5% in the HIV-infected patients (p = 0.32). QFT-GIT and TST respective specificity for active TB in the HIV-uninfected was 25.0% and 57.1% (p = 0.028), and 64.8% and 83.3% in the HIV-infected (p = 0.047). In those with active TB, QFT-GIT results were not associated with microbiological parameters (smear grade, liquid culture status, time-to-positivity of culture) or clinical suspicion of active TB score (provided by the clinicians at enrollment). Combining microbiological tests with both immunological tests significantly increased sensitivity for active TB diagnosis (p = 0.0002), especially in the HIV-infected individuals (p = 0.0016).Conclusion:QFT-GIT and TST have similar diagnostic value for active TB diagnosis. In HIV-infected patients, combining microbiological tests with both immunological tests significantly increases the sensitivity for active TB diagnosis.

AB - Background:The aim of this multicentric prospective study in India was to assess the performance of the QuantiFERON TB-Gold in tube (QFT-GIT), Tuberculin Skin Test (TST) and microbiological results as additional tools for diagnosing active tuberculosis (TB) and latent infection (LTBI) according to Human Immunodeficiency Virus (HIV) status.Methods:Individuals with and without active TB and HIV infection were enrolled between 2006-2008. QFT-GIT and TST results were analyzed per se and in combination with microbiological data.Results:Among the 276 individuals (96 active pulmonary TB and 180 no active TB) tested by QFT-GIT, 18 indeterminate results (6.5%) were found, more significantly numerous in the HIV-infected (15/92; 16.3%) than the HIV-uninfected (3/184; 1.6%)(p<0.0001). QFT-GIT sensitivity for active TB was 82.3% and 92.9% respectively after including or excluding indeterminate results. Clinical sensitivity was significantly lower in the HIV-infected (68.4%) than the HIV-uninfected (91.4%) patients (p = 0.0059). LTBI was detected in 49.3% of subjects without active TB but varied according to TB exposure. When the TST and QFT-GIT were concomitantly performed, the respective sensitivity for active TB diagnosis was 95.0% and 85.0% in the HIV-uninfected (p = 0.60), and 66.7% and 51.5% in the HIV-infected patients (p = 0.32). QFT-GIT and TST respective specificity for active TB in the HIV-uninfected was 25.0% and 57.1% (p = 0.028), and 64.8% and 83.3% in the HIV-infected (p = 0.047). In those with active TB, QFT-GIT results were not associated with microbiological parameters (smear grade, liquid culture status, time-to-positivity of culture) or clinical suspicion of active TB score (provided by the clinicians at enrollment). Combining microbiological tests with both immunological tests significantly increased sensitivity for active TB diagnosis (p = 0.0002), especially in the HIV-infected individuals (p = 0.0016).Conclusion:QFT-GIT and TST have similar diagnostic value for active TB diagnosis. In HIV-infected patients, combining microbiological tests with both immunological tests significantly increases the sensitivity for active TB diagnosis.

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