A small C-terminal sequence of Aurora B is responsible for localization and function

Laetitia Scrittori, Dimitrios A. Skoufias, Fabienne Hans, Véronique Gerson, Paolo Sassone-Corsi, Stefan Dimitrov, Robert L. Margolis

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33 Citations (Scopus)

Abstract

Aurora B, a protein kinase required in mitosis, localizes to inner centromeres at metaphase and the spindle midzone in anaphase and is required for proper chromosome segregation and cytokinesis. Aurora A, a paralogue of Aurora B, localizes instead to centrosomes and spindle microtubules. Except for distinct N termini, Aurora B and Aurora A have highly similar sequences. We have combined small interfering RNA (siRNA) ablation of Aurora B with overexpression of truncation mutants to investigate the role of Aurora B sequence in its function. Reintroduction of Aurora B during siRNA treatment restored its localization and function. This permitted a restoration of function test to determine the sequence requirements for Aurora B targeting and function. Using this rescue protocol, neither N-terminal truncation of Aurora B unique sequence nor substitution with Aurora A N-terminal sequence affected Aurora B localization or function. Truncation of unique Aurora B C-terminal sequence from terminal residue 344 to residue 333 was without effect, but truncation to 326 abolished localization and function. Deletion of residues 326-333 completely abolished localization and blocked cells at prometaphase, establishing this sequence as critical to Aurora B function. Our findings thus establish a small sequence as essential for the distinct localization and function of Aurora B.

Original languageEnglish
Pages (from-to)292-305
Number of pages14
JournalMolecular Biology of the Cell
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 2005
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Scrittori, L., Skoufias, D. A., Hans, F., Gerson, V., Sassone-Corsi, P., Dimitrov, S., & Margolis, R. L. (2005). A small C-terminal sequence of Aurora B is responsible for localization and function. Molecular Biology of the Cell, 16(1), 292-305. https://doi.org/10.1091/mbc.E04-06-0447