A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode

Khaled Mahmoud, John H T Luong

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.

Original languageEnglish
Pages (from-to)1680-1687
Number of pages8
JournalAnalytical Letters
Volume43
Issue number10-11
DOIs
Publication statusPublished - 1 Jul 2010
Externally publishedYes

Fingerprint

Carbon Nanotubes
Single-walled carbon nanotubes (SWCN)
Gold
Nanoparticles
Assays
Electrodes
Peptide Hydrolases
HIV-1
HIV
Atomic Force Microscopy
Voltammetry
Serum
Sulfhydryl Compounds
Atomic force microscopy
Electrons
Scanning
Scanning electron microscopy
Substrates
pepstatin
ferrocene

Keywords

  • Electrochemistry
  • Ferrocene
  • Gold nanoparticle
  • Hiv-1
  • Pepstatin
  • Protease
  • Single-walled carbon nanotube

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry
  • Spectroscopy

Cite this

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