A reproducible sodium-lithium countertransport assay

The outcome of changing key laboratory parameters

Jean B. Smith, Alta L. Price, Roger R. Williams, Wayne M. Hentschel, Wendy Sprowell, Steven Hunt, K. Owen Ash

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

This paper describes experimental conditions for reproducible measurement of sodium-lithium countertransport in red blood cells. The assay is sensitive to temperature (10% per °C at 37°C) and the condition of the red cells; it is affected little by changes in intracellular lithium over the range 2-7 mmol/1 or by red cell concentrations with laboratory hematocrits of 0.03 to 0.07. Repeated measurements of the same subjects give day-to-day coefficients of variation of 10% or less. The mean difference for interlaboratory comparisons is 11%.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalClinica Chimica Acta
Volume122
Issue number3
DOIs
Publication statusPublished - 15 Jul 1982
Externally publishedYes

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Lithium
Assays
Sodium
Cells
Hematocrit
Erythrocytes
Temperature
Blood

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Smith, J. B., Price, A. L., Williams, R. R., Hentschel, W. M., Sprowell, W., Hunt, S., & Ash, K. O. (1982). A reproducible sodium-lithium countertransport assay: The outcome of changing key laboratory parameters. Clinica Chimica Acta, 122(3), 327-335. https://doi.org/10.1016/0009-8981(82)90135-8

A reproducible sodium-lithium countertransport assay : The outcome of changing key laboratory parameters. / Smith, Jean B.; Price, Alta L.; Williams, Roger R.; Hentschel, Wayne M.; Sprowell, Wendy; Hunt, Steven; Ash, K. Owen.

In: Clinica Chimica Acta, Vol. 122, No. 3, 15.07.1982, p. 327-335.

Research output: Contribution to journalArticle

Smith, Jean B. ; Price, Alta L. ; Williams, Roger R. ; Hentschel, Wayne M. ; Sprowell, Wendy ; Hunt, Steven ; Ash, K. Owen. / A reproducible sodium-lithium countertransport assay : The outcome of changing key laboratory parameters. In: Clinica Chimica Acta. 1982 ; Vol. 122, No. 3. pp. 327-335.
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