A reproducible sodium-lithium countertransport assay: The outcome of changing key laboratory parameters

Jean B. Smith, Alta L. Price, Roger R. Williams, Wayne M. Hentschel, Wendy Sprowell, Steven Hunt, K. Owen Ash

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

This paper describes experimental conditions for reproducible measurement of sodium-lithium countertransport in red blood cells. The assay is sensitive to temperature (10% per °C at 37°C) and the condition of the red cells; it is affected little by changes in intracellular lithium over the range 2-7 mmol/1 or by red cell concentrations with laboratory hematocrits of 0.03 to 0.07. Repeated measurements of the same subjects give day-to-day coefficients of variation of 10% or less. The mean difference for interlaboratory comparisons is 11%.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalClinica Chimica Acta
Volume122
Issue number3
DOIs
Publication statusPublished - 15 Jul 1982
Externally publishedYes

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Lithium
Assays
Sodium
Cells
Hematocrit
Erythrocytes
Temperature
Blood

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Smith, J. B., Price, A. L., Williams, R. R., Hentschel, W. M., Sprowell, W., Hunt, S., & Ash, K. O. (1982). A reproducible sodium-lithium countertransport assay: The outcome of changing key laboratory parameters. Clinica Chimica Acta, 122(3), 327-335. https://doi.org/10.1016/0009-8981(82)90135-8

A reproducible sodium-lithium countertransport assay : The outcome of changing key laboratory parameters. / Smith, Jean B.; Price, Alta L.; Williams, Roger R.; Hentschel, Wayne M.; Sprowell, Wendy; Hunt, Steven; Ash, K. Owen.

In: Clinica Chimica Acta, Vol. 122, No. 3, 15.07.1982, p. 327-335.

Research output: Contribution to journalArticle

Smith, Jean B. ; Price, Alta L. ; Williams, Roger R. ; Hentschel, Wayne M. ; Sprowell, Wendy ; Hunt, Steven ; Ash, K. Owen. / A reproducible sodium-lithium countertransport assay : The outcome of changing key laboratory parameters. In: Clinica Chimica Acta. 1982 ; Vol. 122, No. 3. pp. 327-335.
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