A reproducible sodium-lithium countertransport assay: The outcome of changing key laboratory parameters

Jean B. Smith, Alta L. Price, Roger R. Williams, Wayne M. Hentschel, Wendy Sprowell, Steven C. Hunt, K. Owen Ash

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This paper describes experimental conditions for reproducible measurement of sodium-lithium countertransport in red blood cells. The assay is sensitive to temperature (10% per °C at 37°C) and the condition of the red cells; it is affected little by changes in intracellular lithium over the range 2-7 mmol/1 or by red cell concentrations with laboratory hematocrits of 0.03 to 0.07. Repeated measurements of the same subjects give day-to-day coefficients of variation of 10% or less. The mean difference for interlaboratory comparisons is 11%.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalClinica Chimica Acta
Issue number3
Publication statusPublished - 15 Jul 1982


ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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