A reappraisal of the effects of monoclonal antibodies directed at t cell differentiation antigens

Manikkam Suthanthiran, Monica Evelyn, Albert L. Rubin, Kurt H. Stenzel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We examined the effects of monoclonal antibodies (Abs) directed at T3 antigen (expressed on most postthymic T cells), T4antigen (helper/inducer subset) and T8 antigen (suppressor/cytotoxic subset). Anti-T3 induced interleukin-2 production and proliferation of peripheral blood mononuclear cells (PBM). Anti-T4 or T8 did not exhibit such properties. Addition of methylpred-nisolone (MP) or cyclosporine (CsA) to PBM activated with anti-T3 resulted in 79% and 88% suppression of proliferation, respectively. Neither anti-T4 nor anti-T8 mediated significant inhibition of anti-T3-induced proliferation. Primary mixed lymphocyte cultures (MLC) were variably affected by Abs. Anti-T3 augmented proliferation found in primary MLCs aT48 hr and had an inconsistent effect on the proliferative response found at 120–136 hr of culture. Primary cytotoxic T lymphocyte (CTL) generation was consistently suppressed by anti-T3, while natural killer (NK)-cell-like activity was augmented at 72 hr and suppressed after 136 hr of culture. Anti-T4 mediated a dose-dependent suppression of proliferation and CTL generation in the primary MLC and had minimal effect on the induction of NK-cell-like activity. At high concentrations (5000–1000 ng/ml), anti-T8 mediated modest inhibition of proliferation and of the induction of cytolytic activity.Alloimmune memory cells, generated in long-term primary MLCs, were activated by anti-T3to exhibit specific secondary cytolytic activity and NK-cell-like activity in the absence of the original priming stimulus. Neither anti-T nor anti-T, under identical experimental conditions, activated memory cells. When interrelated, our experimental findings indicated that: (1) the ultimate immunity elicited by anti-T antigen interaction is critically dependent upon the immune potential of the cell assessed; (2) MP or CsA can inhibit PBM activation by anti-T; and (3) anti-T4might have a role as an immunosuppressant in renal graft recipients.

Original languageEnglish
Pages (from-to)720-726
Number of pages7
JournalTransplantation
Volume38
Issue number6
DOIs
Publication statusPublished - 1 Jan 1984
Externally publishedYes

Fingerprint

Differentiation Antigens
Cell Differentiation
Monoclonal Antibodies
Natural Killer Cells
Blood Cells
Cytotoxic T-Lymphocytes
Lymphocytes
CD3 Antigens
Viral Tumor Antigens
Immunosuppressive Agents
Helper-Inducer T-Lymphocytes
Cyclosporine
Interleukin-2
Immunity
Transplants
Kidney
Antigens
Thomsen-Friedenreich antibodies
Antibodies

ASJC Scopus subject areas

  • Transplantation

Cite this

A reappraisal of the effects of monoclonal antibodies directed at t cell differentiation antigens. / Suthanthiran, Manikkam; Evelyn, Monica; Rubin, Albert L.; Stenzel, Kurt H.

In: Transplantation, Vol. 38, No. 6, 01.01.1984, p. 720-726.

Research output: Contribution to journalArticle

Suthanthiran, Manikkam ; Evelyn, Monica ; Rubin, Albert L. ; Stenzel, Kurt H. / A reappraisal of the effects of monoclonal antibodies directed at t cell differentiation antigens. In: Transplantation. 1984 ; Vol. 38, No. 6. pp. 720-726.
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