A proteasome inhibitor produced by Burkholderia pseudomallei modulates intracellular growth

Sariqa Wagley, Muthita Vanaporn, Darawan Rinchai, Laura Conejero, Ganjana Lertmemongkolchai, Gregory J. Bancroft, Richard W. Titball

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2 Citations (Scopus)

Abstract

The NRPS/PKS cluster encodes the enzymes necessary for glidobactin synthesis it is partially conserved in various members of the Burkholderia genus including B. pseudomallei. In this study we have shown that the insertional inactivation or deletion of glbC in this cluster in B. pseudomallei could reduce the ability of the bacterium to survive or grow in murine macrophages or in human neutrophils. Exogenously added proteasome inhibitors were able to chemically complement the mutation. The insertional inactivation or deletion of glbC increased virulence in an acute model of infection in Balb/c or C57BL/6 mice but virulence in a chronic model of infection was similar to that of the wild type. Our findings contrast with the previous finding that inactivation of the glb gene cluster in B. pseudomallei strain 1026b resulted in marked attenuation, and provides evidence of differential roles for some genes in virulence of different strains of B. pseudomallei.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalMicrobial Pathogenesis
Volume107
DOIs
Publication statusPublished - 1 Jun 2017
Externally publishedYes

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Keywords

  • Burkholderia
  • Melioidosis
  • Proteasome

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Wagley, S., Vanaporn, M., Rinchai, D., Conejero, L., Lertmemongkolchai, G., Bancroft, G. J., & Titball, R. W. (2017). A proteasome inhibitor produced by Burkholderia pseudomallei modulates intracellular growth. Microbial Pathogenesis, 107, 175-180. https://doi.org/10.1016/j.micpath.2017.03.015