A preliminary study of the short circuit current (Isc) responses of sweat gland cells from normal and anhidrotic horses to purinergic and adrenergic agonists

Darius C S Wilson, Alistair D. Corbett, Cate Steel, Roshni Pannirselvam, Douglas Bovell

Research output: Contribution to journalArticle

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Abstract

The causal factors of equine anhidrosis have not yet been elucidated but defective electrolyte transport mechanisms in the gland are likely to be involved. To investigate this possibility, experiments were performed on cultured equine sweat gland epithelia from five free-sweating UK horses (3 intact males, 2 mares, aged 2-4 years) and from three free-sweating Singapore horses (1 intact male, 2 mares, aged 3-5 years) and three anhidrotic (Singapore) horses (1 intact male, 1 gelding, 1 mare, aged 3-6 years). Cultured cells from each animal were grown on permeable supports and loaded into Ussing chambers to quantify transepithelial resistance and agonist-induced electrolyte transport by the short circuit current (Isc) technique. Transepithelial resistances across the layers of cultured cells were not significantly different between cells from UK and Singapore free-sweating horses, but were significantly reduced in anhidrotic animals. Purinergic agonists added to the apical and basolateral aspects of the cultured cells caused similar increases in Isc between the two populations of unaffected cells, but Isc increases were significantly reduced in anhidrotic animals. Beta-adrenergic agonist stimulation of the anhidrotic cell layers failed to elicit any change in Isc. These pilot results not only confirm earlier conclusions from anatomical findings that failure in the secretory process occurs in anhidrosis but also indicate that both of the known ion transport mechanisms are involved. The trigger for these failures warrants further investigation.

Original languageEnglish
Pages (from-to)152-160
Number of pages9
JournalVeterinary Dermatology
Volume18
Issue number3
DOIs
Publication statusPublished - Jun 2007
Externally publishedYes

Fingerprint

Purinergic Agonists
adrenergic agonists
sweat glands
Sweat Glands
Adrenergic Agonists
Horses
horses
sweating
Sweating
Singapore
Hypohidrosis
cultured cells
mares
Cultured Cells
cells
Electrolytes
agonists
electrolytes
beta-adrenergic agonists
Adrenergic beta-Agonists

ASJC Scopus subject areas

  • veterinary(all)

Cite this

A preliminary study of the short circuit current (Isc) responses of sweat gland cells from normal and anhidrotic horses to purinergic and adrenergic agonists. / Wilson, Darius C S; Corbett, Alistair D.; Steel, Cate; Pannirselvam, Roshni; Bovell, Douglas.

In: Veterinary Dermatology, Vol. 18, No. 3, 06.2007, p. 152-160.

Research output: Contribution to journalArticle

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