A novel phosphorylation-dependent RNase activity of GAP-SH3 binding protein

A potential link between signal transduction and RNA stability

Imed Gallouzi, Fabienne Parker, Karim Chebli, Florence Maurier, Emmanuel Labourier, Isabelle Barlat, Jean Paul Capony, Bruno Tocque, Jamal Tazi

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

A potential p120 GTPase-activating protein (RasGAP) effector, G3BP (RasGAP Src homology 3 [SH3] binding protein), was previously identified based on its ability to bind the SH3 domain of RasGAP. Here er show that G3BP colocalizes and physically interacts with RasGAP at the plasma membrane of serum-stimulated but not quiescent Chinese hamster lung fibroblasts. In quiescent cells, G3BP was hyperphosphorylated on serine residues, and this modification was essential for its activity. Indeed, G3BP harbors a phosphorylation-dependent RNase activity which specifically cleaves the 3'- untranslated region of human c-myc mRNA. The endoribonuclease activity of G3BP can initiate mRNA degradation and therefore represents a link between a RasGAP-mediated signaling pathway and RNA turnover.

Original languageEnglish
Pages (from-to)3956-3965
Number of pages10
JournalMolecular and Cellular Biology
Volume18
Issue number7
Publication statusPublished - Jul 1998
Externally publishedYes

Fingerprint

p120 GTPase Activating Protein
Endoribonucleases
src Homology Domains
RNA Stability
Staphylococcal Protein A
3' Untranslated Regions
Ribonucleases
Cricetulus
Serine
Signal Transduction
Carrier Proteins
Fibroblasts
Phosphorylation
Cell Membrane
RNA
Lung
Messenger RNA
Serum

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

A novel phosphorylation-dependent RNase activity of GAP-SH3 binding protein : A potential link between signal transduction and RNA stability. / Gallouzi, Imed; Parker, Fabienne; Chebli, Karim; Maurier, Florence; Labourier, Emmanuel; Barlat, Isabelle; Capony, Jean Paul; Tocque, Bruno; Tazi, Jamal.

In: Molecular and Cellular Biology, Vol. 18, No. 7, 07.1998, p. 3956-3965.

Research output: Contribution to journalArticle

Gallouzi, I, Parker, F, Chebli, K, Maurier, F, Labourier, E, Barlat, I, Capony, JP, Tocque, B & Tazi, J 1998, 'A novel phosphorylation-dependent RNase activity of GAP-SH3 binding protein: A potential link between signal transduction and RNA stability', Molecular and Cellular Biology, vol. 18, no. 7, pp. 3956-3965.
Gallouzi, Imed ; Parker, Fabienne ; Chebli, Karim ; Maurier, Florence ; Labourier, Emmanuel ; Barlat, Isabelle ; Capony, Jean Paul ; Tocque, Bruno ; Tazi, Jamal. / A novel phosphorylation-dependent RNase activity of GAP-SH3 binding protein : A potential link between signal transduction and RNA stability. In: Molecular and Cellular Biology. 1998 ; Vol. 18, No. 7. pp. 3956-3965.
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