A novel method for the simultaneous enrichment, identification, and quantification of phosphopeptides and sialylated glycopeptides applied to a temporal profile of mouse brain development

Giuseppe Palmisano, Benjamin L. Parker, Kasper Engholm-Keller, Sara Eun Lendal, Katarzyna Kulej, Melanie Schulz, Veit Schwämmle, Mark E. Graham, Henrik Saxtorph, Stuart J. Cordwell, Martin R. Larsen

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

We describe a method that combines an optimized titanium dioxide protocol and hydrophilic interaction liquid chromatography to simultaneously enrich, identify and quantify phosphopeptides and formerly N-linked sialylated glycopeptides to monitor changes associated with cell signaling during mouse brain development. We initially applied the method to enriched membrane fractions from HeLa cells, which allowed the identification of 4468 unique phosphopeptides and 1809 formerly N-linked sialylated glycopeptides. We subsequently combined the method with isobaric tagging for relative quantification to compare changes in phosphopeptide and formerly Nlinked sialylated glycopeptide abundance in the developing mouse brain. A total of 7682 unique phosphopeptide sequences and 3246 unique formerly sialylated glycopeptides were identified. Moreover 669 phosphopeptides and 300 formerly N-sialylated glycopeptides differentially regulated during mouse brain development were detected. This strategy allowed us to reveal extensive changes in post-translational modifications from postnatal mice from day 0 until maturity at day 80. The results of this study confirm the role of sialylation in organ development and provide the first extensive global view of dynamic changes between N-linked sialylation and phosphorylation.

Original languageEnglish
Pages (from-to)1191-1202
Number of pages12
JournalMolecular and Cellular Proteomics
Volume11
Issue number11
DOIs
Publication statusPublished - 1 Nov 2012
Externally publishedYes

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Phosphopeptides
Glycopeptides
Brain
Cell signaling
Phosphorylation
Liquid chromatography
Post Translational Protein Processing
Hydrophobic and Hydrophilic Interactions
HeLa Cells
Liquid Chromatography
Membranes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

A novel method for the simultaneous enrichment, identification, and quantification of phosphopeptides and sialylated glycopeptides applied to a temporal profile of mouse brain development. / Palmisano, Giuseppe; Parker, Benjamin L.; Engholm-Keller, Kasper; Lendal, Sara Eun; Kulej, Katarzyna; Schulz, Melanie; Schwämmle, Veit; Graham, Mark E.; Saxtorph, Henrik; Cordwell, Stuart J.; Larsen, Martin R.

In: Molecular and Cellular Proteomics, Vol. 11, No. 11, 01.11.2012, p. 1191-1202.

Research output: Contribution to journalArticle

Palmisano, Giuseppe ; Parker, Benjamin L. ; Engholm-Keller, Kasper ; Lendal, Sara Eun ; Kulej, Katarzyna ; Schulz, Melanie ; Schwämmle, Veit ; Graham, Mark E. ; Saxtorph, Henrik ; Cordwell, Stuart J. ; Larsen, Martin R. / A novel method for the simultaneous enrichment, identification, and quantification of phosphopeptides and sialylated glycopeptides applied to a temporal profile of mouse brain development. In: Molecular and Cellular Proteomics. 2012 ; Vol. 11, No. 11. pp. 1191-1202.
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