A novel chloride channel localizes to Caenorhabditis elegans spermatids and chloride channel blockers induce spermatid differentiation

Khaled Machaca, Louis J. DeFelice, Steven W. L'Hernault

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Caenorhabditis elegans spermatogenesis is especially suited for studies of nonrandom cytoplasmic segregation during cellular differentiation. Spermatocytes separate from an anuclear cytoplasmic core and undergo two sequential divisions. During the second division, intracellular organelles segregate specifically to spermatids as they bud from an anuclear residual body. We have applied patch-clamp techniques in order to investigate membrane protein distribution during these asymmetric divisions. We show that membrane components, as assayed by voltage-dependent ion channel activity, follow a specific distribution pattern during sperm development. Several voltage-sensitive ion channel activities are observed in spermatocytes and residual bodies, but only a single-channel type can be detected in spermatids, indicating that other channel activities are excluded from or inactivated within these cells as they form. The channel that is observed in spermatids is an inward-rectifying chloride channel (Clir), as indicated by its sensitivity to chloride channel inhibitors and Cl-dependent shifts in its conductance. Treatment of spermatids with Cl channel blockers induce their differentiation into spermatozoa, suggesting that Clir plays a role during this developmental step. These studies are the first application of patch-clamp electrophysiology to C. elegans development.

Original languageEnglish
Pages (from-to)1-16
Number of pages16
JournalDevelopmental Biology
Volume176
Issue number1
DOIs
Publication statusPublished - 25 May 1996
Externally publishedYes

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Chloride Channels
Spermatids
Caenorhabditis elegans
Spermatocytes
Ion Channels
Spermatozoa
Electrophysiology
Patch-Clamp Techniques
Spermatogenesis
Organelles
Membrane Proteins
Membranes

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

A novel chloride channel localizes to Caenorhabditis elegans spermatids and chloride channel blockers induce spermatid differentiation. / Machaca, Khaled; DeFelice, Louis J.; L'Hernault, Steven W.

In: Developmental Biology, Vol. 176, No. 1, 25.05.1996, p. 1-16.

Research output: Contribution to journalArticle

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