A new device for rapid isolation by size and characterization of rare circulating tumor cells

Isabelle Desitter, Bella S. Guerrouahen, Naoual Benali-Furet, Janine Wechsler, Pasi A. Jänne, Yanan Kuang, Masahiko Yanagita, Lilin Wang, Jillian A. Berkowitz, Robert J. Distel, Yvon E. Cayre

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

Background: Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells. Materials and Methods: The ScreenCell® devices are single-use and low-cost innovative devices that use a filter to isolate and sort tumor cells by size. Results: The ScreenCell® Cyto device is able to isolate rare, fixed, tumor cells, with a high recovery rate. Cells are well preserved morphologically. Immunocytochemistry and FISH assays can be performed directly on the filter. The ScreenCell® CC device allows isolation of live cells able to grow in culture. High quality genetic materials can be obtained directly from tumor cells isolated on the ScreenCell® MB device filter. Conclusion: Due to their reduced size, versatility, and capacity to isolate CTCs within minutes, the ScreenCell® devices may be able to simplify and improve non-invasive access to tumor cells.

Original languageEnglish
Pages (from-to)427-441
Number of pages15
JournalAnticancer Research
Volume31
Issue number2
Publication statusPublished - 1 Feb 2011

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Keywords

  • CTCs
  • Cell culture
  • Circulating tumor cells
  • Cytomorphology
  • FISH
  • Lung cancer
  • Microfiltration by size
  • Molecular biology
  • Real-time quantitative reverse transcriptase-polymerase chain reaction
  • qRT-PCR

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Desitter, I., Guerrouahen, B. S., Benali-Furet, N., Wechsler, J., Jänne, P. A., Kuang, Y., Yanagita, M., Wang, L., Berkowitz, J. A., Distel, R. J., & Cayre, Y. E. (2011). A new device for rapid isolation by size and characterization of rare circulating tumor cells. Anticancer Research, 31(2), 427-441.