A new device for rapid isolation by size and characterization of rare circulating tumor cells

Isabelle Desitter, Bella S. Guerrouahen, Naoual Benali-Furet, Janine Wechsler, Pasi A. Jänne, Yanan Kuang, Masahiko Yanagita, Lilin Wang, Jillian A. Berkowitz, Robert J. Distel, Yvon E. Cayre

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

Background: Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells. Materials and Methods: The ScreenCell® devices are single-use and low-cost innovative devices that use a filter to isolate and sort tumor cells by size. Results: The ScreenCell® Cyto device is able to isolate rare, fixed, tumor cells, with a high recovery rate. Cells are well preserved morphologically. Immunocytochemistry and FISH assays can be performed directly on the filter. The ScreenCell® CC device allows isolation of live cells able to grow in culture. High quality genetic materials can be obtained directly from tumor cells isolated on the ScreenCell® MB device filter. Conclusion: Due to their reduced size, versatility, and capacity to isolate CTCs within minutes, the ScreenCell® devices may be able to simplify and improve non-invasive access to tumor cells.

Original languageEnglish
Pages (from-to)427-441
Number of pages15
JournalAnticancer Research
Volume31
Issue number2
Publication statusPublished - 1 Feb 2011
Externally publishedYes

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Circulating Neoplastic Cells
Equipment and Supplies
Neoplasms
Cell Separation
Cell Size
Clone Cells
Immunohistochemistry
Costs and Cost Analysis
Genes

Keywords

  • Cell culture
  • Circulating tumor cells
  • CTCs
  • Cytomorphology
  • FISH
  • Lung cancer
  • Microfiltration by size
  • Molecular biology
  • qRT-PCR
  • Real-time quantitative reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Desitter, I., Guerrouahen, B. S., Benali-Furet, N., Wechsler, J., Jänne, P. A., Kuang, Y., ... Cayre, Y. E. (2011). A new device for rapid isolation by size and characterization of rare circulating tumor cells. Anticancer Research, 31(2), 427-441.

A new device for rapid isolation by size and characterization of rare circulating tumor cells. / Desitter, Isabelle; Guerrouahen, Bella S.; Benali-Furet, Naoual; Wechsler, Janine; Jänne, Pasi A.; Kuang, Yanan; Yanagita, Masahiko; Wang, Lilin; Berkowitz, Jillian A.; Distel, Robert J.; Cayre, Yvon E.

In: Anticancer Research, Vol. 31, No. 2, 01.02.2011, p. 427-441.

Research output: Contribution to journalArticle

Desitter, I, Guerrouahen, BS, Benali-Furet, N, Wechsler, J, Jänne, PA, Kuang, Y, Yanagita, M, Wang, L, Berkowitz, JA, Distel, RJ & Cayre, YE 2011, 'A new device for rapid isolation by size and characterization of rare circulating tumor cells', Anticancer Research, vol. 31, no. 2, pp. 427-441.
Desitter I, Guerrouahen BS, Benali-Furet N, Wechsler J, Jänne PA, Kuang Y et al. A new device for rapid isolation by size and characterization of rare circulating tumor cells. Anticancer Research. 2011 Feb 1;31(2):427-441.
Desitter, Isabelle ; Guerrouahen, Bella S. ; Benali-Furet, Naoual ; Wechsler, Janine ; Jänne, Pasi A. ; Kuang, Yanan ; Yanagita, Masahiko ; Wang, Lilin ; Berkowitz, Jillian A. ; Distel, Robert J. ; Cayre, Yvon E. / A new device for rapid isolation by size and characterization of rare circulating tumor cells. In: Anticancer Research. 2011 ; Vol. 31, No. 2. pp. 427-441.
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