A mitogen-activated protein kinase regulates male gametogenesis and transmission of the malaria parasite Plasmodium berghei

Radha Rangarajan, Amy K. Bei, Deepa Jethwaney, Priscilla Maldonado, Dominique Dorin, Ali Sultan, Christian Doerig

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Differentiation of malaria parasites into sexual forms (gametocytes) in the vertebrate host and their subsequent development into gametes in the mosquito vector are crucial steps in the completion of the parasite's life cycle and transmission of the disease. The molecular mechanisms that regulate the sexual cycle are poorly understood. Although several signal transduction pathways have been implicated, a clear understanding of the pathways involved has yet to emerge. Here, we show that a Plasmodium berghei homologue of Plasmodium falciparum mitogen-activated kinase-2 (Pfmap-2), a gametocyte-specific mitogen-activated protein kinase (MAPK), is required for male gamete formation. Parasites lacking Pbmap-2 are competent for gametocytogenesis, but exflagellation of male gametocytes, the process that leads to male gamete formation, is almost entirely abolished in mutant parasites. Consistent with this result, transmission of mutant parasites to mosquitoes is grossly impaired. This finding identifies a crucial role for a MAPK pathway in malaria transmission.

Original languageEnglish
Pages (from-to)464-469
Number of pages6
JournalEMBO Reports
Volume6
Issue number5
DOIs
Publication statusPublished - May 2005
Externally publishedYes

Fingerprint

Gametogenesis
Plasmodium berghei
Mitogen-Activated Protein Kinases
Malaria
Parasites
Germ Cells
Signal transduction
Plasmodium falciparum
Life Cycle Stages
Culicidae
Mitogens
Vertebrates
Life cycle
Signal Transduction
Phosphotransferases

Keywords

  • Exflagellation
  • Gametogenesis
  • MAPK
  • Plasmodium
  • Transmission

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

A mitogen-activated protein kinase regulates male gametogenesis and transmission of the malaria parasite Plasmodium berghei. / Rangarajan, Radha; Bei, Amy K.; Jethwaney, Deepa; Maldonado, Priscilla; Dorin, Dominique; Sultan, Ali; Doerig, Christian.

In: EMBO Reports, Vol. 6, No. 5, 05.2005, p. 464-469.

Research output: Contribution to journalArticle

Rangarajan, Radha ; Bei, Amy K. ; Jethwaney, Deepa ; Maldonado, Priscilla ; Dorin, Dominique ; Sultan, Ali ; Doerig, Christian. / A mitogen-activated protein kinase regulates male gametogenesis and transmission of the malaria parasite Plasmodium berghei. In: EMBO Reports. 2005 ; Vol. 6, No. 5. pp. 464-469.
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