A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton

Henning Horn, Dae In Kim, Graham D. Wright, Esther Sook Miin Wong, Colin L. Stewart, Brian Burke, Kyle J. Roux

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Chromosome pairing is an essential meiotic event that ensures faithful haploidization and recombination of the genome. Pairing of homologous chromosomes is facilitated by telomere-led chromosome movements and formation of a meiotic bouquet, where telomeres cluster to one pole of the nucleus. In metazoans, telomere clustering is dynein and microtubule dependent and requires Sun1, an inner nuclear membrane protein. Here we provide a functional analysis of KASH5, a mammalian dynein-binding protein of the outer nuclear membrane that forms a meiotic complex with Sun1. This protein is related to zebrafish futile cycle (Fue), a nuclear envelope (NE) constituent required for pronuclear migration. Mice deficient in this Fue homologue are infertile. Males display meiotic arrest in which pairing of homologous chromosomes fails. These findings demonstrate that telomere attachment to the NE is insufficient to promote pairing and that telomere attachment sites must be coupled to cytoplasmic dynein and the microtubule system to ensure meiotic progression.

Original languageEnglish
Pages (from-to)1023-1039
Number of pages17
JournalJournal of Cell Biology
Volume202
Issue number7
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Telomere
Cytoskeleton
Nuclear Envelope
Chromosome Pairing
Chromosomes
Dyneins
Microtubules
Substrate Cycling
Cytoplasmic Dyneins
Zebrafish
Nuclear Proteins
Genetic Recombination
Cluster Analysis
Carrier Proteins
Membrane Proteins
Protein Domains
Genome
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Horn, H., Kim, D. I., Wright, G. D., Wong, E. S. M., Stewart, C. L., Burke, B., & Roux, K. J. (2013). A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton. Journal of Cell Biology, 202(7), 1023-1039. https://doi.org/10.1083/jcb.201304004

A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton. / Horn, Henning; Kim, Dae In; Wright, Graham D.; Wong, Esther Sook Miin; Stewart, Colin L.; Burke, Brian; Roux, Kyle J.

In: Journal of Cell Biology, Vol. 202, No. 7, 2013, p. 1023-1039.

Research output: Contribution to journalArticle

Horn, H, Kim, DI, Wright, GD, Wong, ESM, Stewart, CL, Burke, B & Roux, KJ 2013, 'A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton', Journal of Cell Biology, vol. 202, no. 7, pp. 1023-1039. https://doi.org/10.1083/jcb.201304004
Horn, Henning ; Kim, Dae In ; Wright, Graham D. ; Wong, Esther Sook Miin ; Stewart, Colin L. ; Burke, Brian ; Roux, Kyle J. / A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton. In: Journal of Cell Biology. 2013 ; Vol. 202, No. 7. pp. 1023-1039.
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