A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24

Angélique Biancotto, Beda Brichacek, Silvia S. Chen, Wendy Fitzgerald, Andrea Lisco, Christophe Vanpouille, Leonid Margolis, Jean-Charles B. Grivel

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Nucleic acid measurements are used to follow HIV-1 viral load in clinical applications while p24 ELISA is commonly used to monitor HIV-1 replication in research settings. Current ELISA assays are expensive and offer a narrow dynamic measurement range. This report describes a simple, sensitive and inexpensive bead-based assay offering a wide dynamic measurement range. This cytometric bead assay allows the detection of p24 concentrations over 4 orders of magnitude from less than 0.4 pg to up to 20,000 pg ml-1 in a volume of 50 μl and can be combined with other measurements.

Original languageEnglish
Pages (from-to)98-101
Number of pages4
JournalJournal of Virological Methods
Volume157
Issue number1
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Fingerprint

HIV-1
Enzyme-Linked Immunosorbent Assay
Viral Load
Nucleic Acids
Research

Keywords

  • Detection
  • Diagnosis
  • HIV-1
  • Multiplex assay
  • p24

ASJC Scopus subject areas

  • Virology

Cite this

A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24. / Biancotto, Angélique; Brichacek, Beda; Chen, Silvia S.; Fitzgerald, Wendy; Lisco, Andrea; Vanpouille, Christophe; Margolis, Leonid; Grivel, Jean-Charles B.

In: Journal of Virological Methods, Vol. 157, No. 1, 04.2009, p. 98-101.

Research output: Contribution to journalArticle

Biancotto, A, Brichacek, B, Chen, SS, Fitzgerald, W, Lisco, A, Vanpouille, C, Margolis, L & Grivel, J-CB 2009, 'A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24', Journal of Virological Methods, vol. 157, no. 1, pp. 98-101. https://doi.org/10.1016/j.jviromet.2008.11.013
Biancotto, Angélique ; Brichacek, Beda ; Chen, Silvia S. ; Fitzgerald, Wendy ; Lisco, Andrea ; Vanpouille, Christophe ; Margolis, Leonid ; Grivel, Jean-Charles B. / A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24. In: Journal of Virological Methods. 2009 ; Vol. 157, No. 1. pp. 98-101.
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