A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24

Angélique Biancotto, Beda Brichacek, Silvia S. Chen, Wendy Fitzgerald, Andrea Lisco, Christophe Vanpouille, Leonid Margolis, Jean-Charles B. Grivel

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Nucleic acid measurements are used to follow HIV-1 viral load in clinical applications while p24 ELISA is commonly used to monitor HIV-1 replication in research settings. Current ELISA assays are expensive and offer a narrow dynamic measurement range. This report describes a simple, sensitive and inexpensive bead-based assay offering a wide dynamic measurement range. This cytometric bead assay allows the detection of p24 concentrations over 4 orders of magnitude from less than 0.4 pg to up to 20,000 pg ml-1 in a volume of 50 μl and can be combined with other measurements.

Original languageEnglish
Pages (from-to)98-101
Number of pages4
JournalJournal of Virological Methods
Volume157
Issue number1
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

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Keywords

  • Detection
  • Diagnosis
  • HIV-1
  • Multiplex assay
  • p24

ASJC Scopus subject areas

  • Virology

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