A high-resolution physical map of human chromosome 21p using yeast artificial chromosomes

Sheng Yue Wang, Marc Cruts, Jurgen Del-Favero, Yi Zhang, Fadel Tissir, Marie Claude Potier, David Patterson, Dean Nizetic, Assumpció Bosch, Haiming Chen, Lynda Bennett, Xavier Estivill, Anna Kessling, Stylianos E. Antonarakis, Christine Van Broeckhoven

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The short arm of human chromosome 21 (21p) contains many different types of repetitive sequences and is highly homologous to the short arms of other acrocentric chromosomes. Owing to its repetitive nature and the lack of chromosome 21p-specific molecular markers, most physical maps of chromosome 21 exclude this region. We constructed a physical map of chromosome 21p using sequence tagged site (STS) content mapping of yeast artificial chromosomes (YACs). To this end, 39 STSs located on the short arm or near the centromere of chromosome 21 were constructed, including four polymorphic simple tandem repeats (STRs) and two expressed sequence tags (ESTs). Thirty YACs were selected from the St. Louis YAC library, the chromosome 21-enriched ICRF YAC library, and the CEPH YAC and megaYAC libraries. These were assembled in a YAC contig map ranging from the centromere to the rDNA gene cluster at 21p12. The total size of the region covered by YACs is estimated between 2.9 and 5 Mb. The integrity of the YAC contig was confirmed by restriction enzyme fingerprinting and fluorescence in situ hybridization (FISH). One gap with an estimated size of 400 kb remained near the telomeric end of the contig. This YAC contig map of the short arm of human chromosome 21 constitutes a basic framework for further structural and functional studies of chromosome 21p.

Original languageEnglish
Pages (from-to)1059-1073
Number of pages15
JournalGenome Research
Volume9
Issue number11
DOIs
Publication statusPublished - 1 Nov 1999

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Wang, S. Y., Cruts, M., Del-Favero, J., Zhang, Y., Tissir, F., Potier, M. C., Patterson, D., Nizetic, D., Bosch, A., Chen, H., Bennett, L., Estivill, X., Kessling, A., Antonarakis, S. E., & Van Broeckhoven, C. (1999). A high-resolution physical map of human chromosome 21p using yeast artificial chromosomes. Genome Research, 9(11), 1059-1073. https://doi.org/10.1101/gr.9.11.1059