A genome annotation-driven approach to cloning the human ORFeome.

John E. Collins, Charmain L. Wright, Carol A. Edwards, Matthew P. Davis, James A. Grinham, Charlotte G. Cole, Melanie E. Goward, Begoña Aguado, Meera Mallya, Younes Mokrab, Elizabeth J. Huckle, David M. Beare, Ian Dunham

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.

Original languageEnglish
JournalGenome Biology
Volume5
Issue number10
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Organism Cloning
clone
molecular cloning
genome
Complementary DNA
Clone Cells
Genome
clones
Open Reading Frames
open reading frames
Chromosomes, Human, Pair 22
gene
Human Chromosomes
Genes
chromosome
genomics
genes
chromosomes
Polymerase Chain Reaction
cloning

ASJC Scopus subject areas

  • Genetics

Cite this

Collins, J. E., Wright, C. L., Edwards, C. A., Davis, M. P., Grinham, J. A., Cole, C. G., ... Dunham, I. (2004). A genome annotation-driven approach to cloning the human ORFeome. Genome Biology, 5(10).

A genome annotation-driven approach to cloning the human ORFeome. / Collins, John E.; Wright, Charmain L.; Edwards, Carol A.; Davis, Matthew P.; Grinham, James A.; Cole, Charlotte G.; Goward, Melanie E.; Aguado, Begoña; Mallya, Meera; Mokrab, Younes; Huckle, Elizabeth J.; Beare, David M.; Dunham, Ian.

In: Genome Biology, Vol. 5, No. 10, 2004.

Research output: Contribution to journalArticle

Collins, JE, Wright, CL, Edwards, CA, Davis, MP, Grinham, JA, Cole, CG, Goward, ME, Aguado, B, Mallya, M, Mokrab, Y, Huckle, EJ, Beare, DM & Dunham, I 2004, 'A genome annotation-driven approach to cloning the human ORFeome.', Genome Biology, vol. 5, no. 10.
Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG et al. A genome annotation-driven approach to cloning the human ORFeome. Genome Biology. 2004;5(10).
Collins, John E. ; Wright, Charmain L. ; Edwards, Carol A. ; Davis, Matthew P. ; Grinham, James A. ; Cole, Charlotte G. ; Goward, Melanie E. ; Aguado, Begoña ; Mallya, Meera ; Mokrab, Younes ; Huckle, Elizabeth J. ; Beare, David M. ; Dunham, Ian. / A genome annotation-driven approach to cloning the human ORFeome. In: Genome Biology. 2004 ; Vol. 5, No. 10.
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