A genome annotation-driven approach to cloning the human ORFeome.

John E. Collins, Charmain L. Wright, Carol A. Edwards, Matthew P. Davis, James A. Grinham, Charlotte G. Cole, Melanie E. Goward, Begoña Aguado, Meera Mallya, Younes Mokrab, Elizabeth J. Huckle, David M. Beare, Ian Dunham

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24 Citations (Scopus)

Abstract

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.

Original languageEnglish
Pages (from-to)R84
JournalGenome biology
Volume5
Issue number10
Publication statusPublished - 2004

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ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

Cite this

Collins, J. E., Wright, C. L., Edwards, C. A., Davis, M. P., Grinham, J. A., Cole, C. G., Goward, M. E., Aguado, B., Mallya, M., Mokrab, Y., Huckle, E. J., Beare, D. M., & Dunham, I. (2004). A genome annotation-driven approach to cloning the human ORFeome. Genome biology, 5(10), R84.