A comparison of proliferation indices in human anterior pituitary adenomas using formalin-fixed tissue and in vitro cell culture

Stephen Atkin, Victoria L. Green, Leslie J. Hipkin, Alex M. Landolt, Patrick M. Foy, Richard V. Jeffreys, Michael C. White

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The authors compared detection methods for cell proliferation in human anterior pituitary adenomas using histological sections and dispersed cell culture. After tumor cells had been grown for 4 days in dispersed culture, bromodeoxyuridine (BUdR), proliferating cell nuclear antigen (PCNA), and Ki- 67 were compared by double immunostaining and contrasted with single staining of PCNA and Ki-67 indices in the corresponding histological sections from 12 human pituitary adenomas. In vitro, the BUdR labeling index was positive in six of 12 tumors (range < 0.1-5, 1%), 10 of 12 tumors were PCNA-positive (range < 0.1-100%), and Ki-67 was positive in 10 of 12 adenomas (range < 0.1- 8%). In vitro, BUdR and Ki-67 gave similar proliferative indices for 10 of 12 adenomas. In vivo, the PCNA labeling index was positive in 12 of 12 adenomas (range 0.9-95%) and Ki-67 was positive in 11 of 12 adenomas (range < 0.1- 2%). Tumors with a labeling index less than 0.1% were considered to be negative for proliferation. High PCNA values were found in vitro and in vivo, whereas Ki-67 labeling indices were similar in vitro and in vivo for nine of 12 adenomas. It is concluded that Ki-67 proliferative indices in vivo infect those found in vitro, at least after 4 days in dispersed culture, but that PCNA overestimates pituitary adenoma proliferation in histological sections as well as in dispersed culture.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalJournal of Neurosurgery
Volume87
Issue number1
Publication statusPublished - Jul 1997
Externally publishedYes

Fingerprint

Proliferating Cell Nuclear Antigen
Pituitary Neoplasms
Formaldehyde
Adenoma
Cell Culture Techniques
Bromodeoxyuridine
Neoplasms
In Vitro Techniques
Cell Proliferation
Staining and Labeling

Keywords

  • Human anterior pituitary tumor
  • Ki-67
  • MIB-1 bromodeoxyuridine
  • Proliferating cell nuclear antigen

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Atkin, S., Green, V. L., Hipkin, L. J., Landolt, A. M., Foy, P. M., Jeffreys, R. V., & White, M. C. (1997). A comparison of proliferation indices in human anterior pituitary adenomas using formalin-fixed tissue and in vitro cell culture. Journal of Neurosurgery, 87(1), 85-88.

A comparison of proliferation indices in human anterior pituitary adenomas using formalin-fixed tissue and in vitro cell culture. / Atkin, Stephen; Green, Victoria L.; Hipkin, Leslie J.; Landolt, Alex M.; Foy, Patrick M.; Jeffreys, Richard V.; White, Michael C.

In: Journal of Neurosurgery, Vol. 87, No. 1, 07.1997, p. 85-88.

Research output: Contribution to journalArticle

Atkin, S, Green, VL, Hipkin, LJ, Landolt, AM, Foy, PM, Jeffreys, RV & White, MC 1997, 'A comparison of proliferation indices in human anterior pituitary adenomas using formalin-fixed tissue and in vitro cell culture', Journal of Neurosurgery, vol. 87, no. 1, pp. 85-88.
Atkin, Stephen ; Green, Victoria L. ; Hipkin, Leslie J. ; Landolt, Alex M. ; Foy, Patrick M. ; Jeffreys, Richard V. ; White, Michael C. / A comparison of proliferation indices in human anterior pituitary adenomas using formalin-fixed tissue and in vitro cell culture. In: Journal of Neurosurgery. 1997 ; Vol. 87, No. 1. pp. 85-88.
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