A comparison of proliferation indices in human anterior pituitary adenomas using formalin-fixed tissue and in vitro cell culture

Stephen Atkin, Victoria L. Green, Leslie J. Hipkin, Alex M. Landolt, Patrick M. Foy, Richard V. Jeffreys, Michael C. White

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Abstract

The authors compared detection methods for cell proliferation in human anterior pituitary adenomas using histological sections and dispersed cell culture. After tumor cells had been grown for 4 days in dispersed culture, bromodeoxyuridine (BUdR), proliferating cell nuclear antigen (PCNA), and Ki- 67 were compared by double immunostaining and contrasted with single staining of PCNA and Ki-67 indices in the corresponding histological sections from 12 human pituitary adenomas. In vitro, the BUdR labeling index was positive in six of 12 tumors (range < 0.1-5, 1%), 10 of 12 tumors were PCNA-positive (range < 0.1-100%), and Ki-67 was positive in 10 of 12 adenomas (range < 0.1- 8%). In vitro, BUdR and Ki-67 gave similar proliferative indices for 10 of 12 adenomas. In vivo, the PCNA labeling index was positive in 12 of 12 adenomas (range 0.9-95%) and Ki-67 was positive in 11 of 12 adenomas (range < 0.1- 2%). Tumors with a labeling index less than 0.1% were considered to be negative for proliferation. High PCNA values were found in vitro and in vivo, whereas Ki-67 labeling indices were similar in vitro and in vivo for nine of 12 adenomas. It is concluded that Ki-67 proliferative indices in vivo infect those found in vitro, at least after 4 days in dispersed culture, but that PCNA overestimates pituitary adenoma proliferation in histological sections as well as in dispersed culture.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalJournal of Neurosurgery
Volume87
Issue number1
Publication statusPublished - Jul 1997
Externally publishedYes

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Keywords

  • Human anterior pituitary tumor
  • Ki-67
  • MIB-1 bromodeoxyuridine
  • Proliferating cell nuclear antigen

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Atkin, S., Green, V. L., Hipkin, L. J., Landolt, A. M., Foy, P. M., Jeffreys, R. V., & White, M. C. (1997). A comparison of proliferation indices in human anterior pituitary adenomas using formalin-fixed tissue and in vitro cell culture. Journal of Neurosurgery, 87(1), 85-88.