A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes

Samir Kelkar, P. De Bishnu, Guangping Gao, James M. Wilson, Ronald Crystal, Philip L. Leopold

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Binding of AAV serotype 2 (AAV2) was enhanced in a nucleotide-dependent manner by the presence of total cellular microtubule-associated proteins (MAPs) but not cytoplasmic dynein-depleted MAPs. Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses.

Original languageEnglish
Pages (from-to)7781-7785
Number of pages5
JournalJournal of Virology
Volume80
Issue number15
DOIs
Publication statusPublished - 1 Aug 2006
Externally publishedYes

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Dependovirus
Cytoplasmic Dyneins
Adenoviridae
Satellite Viruses
Microtubules
microtubules
serotypes
viruses
Microtubule-Associated Proteins
Viral Genome
Capsid Proteins
coat proteins
Nucleotides
Binding Sites
Serogroup
binding sites
Viruses
proteins
nucleotides
Infection

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes. / Kelkar, Samir; De Bishnu, P.; Gao, Guangping; Wilson, James M.; Crystal, Ronald; Leopold, Philip L.

In: Journal of Virology, Vol. 80, No. 15, 01.08.2006, p. 7781-7785.

Research output: Contribution to journalArticle

Kelkar, Samir ; De Bishnu, P. ; Gao, Guangping ; Wilson, James M. ; Crystal, Ronald ; Leopold, Philip L. / A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes. In: Journal of Virology. 2006 ; Vol. 80, No. 15. pp. 7781-7785.
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