3D structure of fish muscle myosin filaments

Felicity Eakins, Hind A. Al-Khayat, Robert W. Kensler, Ed P. Morris, John M. Squire

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Myosin filaments isolated from goldfish (Carassius auratus) muscle under relaxing conditions and viewed in negative stain by electron microscopy have been subjected to 3D helical reconstruction to provide details of the myosin head arrangement in relaxed muscle. Previous X-ray diffraction studies of fish muscle (plaice) myosin filaments have suggested that the heads project a long way from the filament surface rather than lying down flat and that heads in a single myosin molecule tend to interact with each other rather than with heads from adjacent molecules. Evidence has also been presented that the head tilt is away from the M-band. Here we seek to confirm these conclusions using a totally independent method. By using 3D helical reconstruction of isolated myosin filaments the known perturbation of the head array in vertebrate muscles was inevitably averaged out. The 3D reconstruction was therefore compared with the X-ray model after it too had been helically averaged. The resulting images showed the same characteristic features: heads projecting out from the filament backbone to high radius and the motor domains at higher radius and further away from the M-band than the light-chain-binding neck domains (lever arms) of the heads.

Original languageEnglish
Pages (from-to)154-163
Number of pages10
JournalJournal of Structural Biology
Volume137
Issue number1-2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Myosins
Fishes
Head
Muscles
Goldfish
Flounder
X-Ray Diffraction
Vertebrates
Electron Microscopy
Coloring Agents
Neck
X-Rays
Light

ASJC Scopus subject areas

  • Structural Biology

Cite this

Eakins, F., Al-Khayat, H. A., Kensler, R. W., Morris, E. P., & Squire, J. M. (2002). 3D structure of fish muscle myosin filaments. Journal of Structural Biology, 137(1-2), 154-163. https://doi.org/10.1006/jsbi.2002.4453

3D structure of fish muscle myosin filaments. / Eakins, Felicity; Al-Khayat, Hind A.; Kensler, Robert W.; Morris, Ed P.; Squire, John M.

In: Journal of Structural Biology, Vol. 137, No. 1-2, 2002, p. 154-163.

Research output: Contribution to journalArticle

Eakins, F, Al-Khayat, HA, Kensler, RW, Morris, EP & Squire, JM 2002, '3D structure of fish muscle myosin filaments', Journal of Structural Biology, vol. 137, no. 1-2, pp. 154-163. https://doi.org/10.1006/jsbi.2002.4453
Eakins F, Al-Khayat HA, Kensler RW, Morris EP, Squire JM. 3D structure of fish muscle myosin filaments. Journal of Structural Biology. 2002;137(1-2):154-163. https://doi.org/10.1006/jsbi.2002.4453
Eakins, Felicity ; Al-Khayat, Hind A. ; Kensler, Robert W. ; Morris, Ed P. ; Squire, John M. / 3D structure of fish muscle myosin filaments. In: Journal of Structural Biology. 2002 ; Vol. 137, No. 1-2. pp. 154-163.
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