3D culture model and computer-assisted videomicroscopy to analyze migratory behavior of noninvasive and invasive bronchial epithelial cells

Salma Hazgui, Noël Bonnet, Jérôme Cutrona, Béatrice Nawrocki-Raby, Myriam Polette, Lotfi Chouchane, Philippe Birembaut, Jean Marie Zahm

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

To date, most of the studies in the field of cell migration have been applied to two-dimensional (2D) models. To mimic the three-dimensional (3D) conditions similar to those observed in vivo during tumor invasion, we developed a 3D model of cell migration in which cells were embedded in a collagen 1 matrix placed in a double-compartment chamber. Using time-lapse videomicroscopy and interactive cell tracking in a four-dimensional data set, we determined the cell trajectories and their migration kinetics. We compared the 2D and 3D migratory behavior of a noninvasive cell line (16HBE) with the migratory behavior of an invasive cell line (BZR). Our results show that the 3D migration kinetics of the noninvasive cell line were lower than the migration kinetics of the invasive cell line. In contrast, in 2D models, no significant difference was observed between the two cell lines. To validate our 3D model, we further investigated the effect of epidermal growth factor (EGF), a promoter of tumor cell motility and invasion on the noninvasive cell line (16HBE). EGF increased significantly the migration kinetics of the noninvasive cell line. Our results show that the 3D model of cell migration allowed us to differentiate the migratory behavior of invasive and noninvasive cells and that such a model can help in the development of molecular targeted therapy as it approaches the in vivo conditions.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume289
Issue number6 58-6
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

Fingerprint

Video Microscopy
Computer Simulation
Epithelial Cells
Cells
Cell Line
Cell Movement
Kinetics
Epidermal Growth Factor
Cell Tracking
Molecular Targeted Therapy
Carcinogens
Collagen
Tumors
Trajectories

Keywords

  • Epidermal growth factor
  • Image analysis
  • Kinetic migration
  • Metastasis
  • Tumor invasion

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

3D culture model and computer-assisted videomicroscopy to analyze migratory behavior of noninvasive and invasive bronchial epithelial cells. / Hazgui, Salma; Bonnet, Noël; Cutrona, Jérôme; Nawrocki-Raby, Béatrice; Polette, Myriam; Chouchane, Lotfi; Birembaut, Philippe; Zahm, Jean Marie.

In: American Journal of Physiology - Cell Physiology, Vol. 289, No. 6 58-6, 12.2005.

Research output: Contribution to journalArticle

Hazgui, Salma ; Bonnet, Noël ; Cutrona, Jérôme ; Nawrocki-Raby, Béatrice ; Polette, Myriam ; Chouchane, Lotfi ; Birembaut, Philippe ; Zahm, Jean Marie. / 3D culture model and computer-assisted videomicroscopy to analyze migratory behavior of noninvasive and invasive bronchial epithelial cells. In: American Journal of Physiology - Cell Physiology. 2005 ; Vol. 289, No. 6 58-6.
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