3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet

Elena Silvestri, Rosalba Senese, Federica Cioffi, Rita De Matteis, Davide Lattanzi, Assunta Lombardi, Antonia Giacco, Anna Maria Salzano, Andrea Scaloni, Michele Ceccarelli, Maria Moreno, Fernando Goglia, Antonia Lanni, Pieter de Lange

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

When administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 μg/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white adipose tissue (VAT) contributed to the beneficial effect of 3,5-T2 over time (between 1 day and 4 weeks following administration). 3,5-T2 programmed the adipocyte for lipolysis by rapidly inducing hormone sensitive lipase (HSL) phosphorylation at the protein kinase A-responsive site Ser563, accompanied with glycerol release at the 1-week time-point, contributing to the partial normalization of adipocyte volume with respect to control (N) animals. After two weeks, when the adipocyte volumes of HFD-3,5-T2 rats were completely normalized to those of the controls (N), 3,5-T2 consistently induced HSL phosphorylation at Ser563, indicative of a combined effect of 3,5-T2-induced adipose lipolysis and increasing non-adipose oxidative metabolism. VAT proteome analysis after 4 weeks of treatment revealed that 3,5-T2 significantly altered the proteomic profile of HFD rats and produced a marked pro-angiogenic action. This was associated with a reduced representation of proteins involved in lipid storage or related to response to oxidative stress, and a normalization of the levels of those involved in lipogenesis-associated mitochondrial function. In conclusion, the prevention of VAT mass-gain by 3,5-T2 occurred through different molecular pathways that, together with the previously reported stimulation of resting metabolism and liver fatty acid oxidation, are associated with an anti adipogenic/lipogenic potential and positively impact on tissue health.

Original languageEnglish
JournalNutrients
Volume11
Issue number2
DOIs
Publication statusPublished - 27 Jan 2019
Externally publishedYes

Fingerprint

thyronine
Intra-Abdominal Fat
High Fat Diet
high fat diet
white adipose tissue
adipose tissue
adipocytes
rats
lipolysis
phosphorylation
White Adipose Tissue
hormones
Adipocytes
Sterol Esterase
cAMP-dependent protein kinase
beta oxidation
aerobiosis
lipogenesis
adiposity
proteome

Keywords

  • 3,5-diiodo-L-thyronine
  • ATGL
  • hormone sensitive lipase
  • lipolysis
  • proteomics
  • visceral white adipose tissue

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Silvestri, E., Senese, R., Cioffi, F., De Matteis, R., Lattanzi, D., Lombardi, A., ... de Lange, P. (2019). 3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet. Nutrients, 11(2). https://doi.org/10.3390/nu11020278

3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet. / Silvestri, Elena; Senese, Rosalba; Cioffi, Federica; De Matteis, Rita; Lattanzi, Davide; Lombardi, Assunta; Giacco, Antonia; Salzano, Anna Maria; Scaloni, Andrea; Ceccarelli, Michele; Moreno, Maria; Goglia, Fernando; Lanni, Antonia; de Lange, Pieter.

In: Nutrients, Vol. 11, No. 2, 27.01.2019.

Research output: Contribution to journalArticle

Silvestri, E, Senese, R, Cioffi, F, De Matteis, R, Lattanzi, D, Lombardi, A, Giacco, A, Salzano, AM, Scaloni, A, Ceccarelli, M, Moreno, M, Goglia, F, Lanni, A & de Lange, P 2019, '3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet', Nutrients, vol. 11, no. 2. https://doi.org/10.3390/nu11020278
Silvestri, Elena ; Senese, Rosalba ; Cioffi, Federica ; De Matteis, Rita ; Lattanzi, Davide ; Lombardi, Assunta ; Giacco, Antonia ; Salzano, Anna Maria ; Scaloni, Andrea ; Ceccarelli, Michele ; Moreno, Maria ; Goglia, Fernando ; Lanni, Antonia ; de Lange, Pieter. / 3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet. In: Nutrients. 2019 ; Vol. 11, No. 2.
@article{62f691229ed84b98be2e6e9399bd8ba5,
title = "3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet",
abstract = "When administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 μg/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white adipose tissue (VAT) contributed to the beneficial effect of 3,5-T2 over time (between 1 day and 4 weeks following administration). 3,5-T2 programmed the adipocyte for lipolysis by rapidly inducing hormone sensitive lipase (HSL) phosphorylation at the protein kinase A-responsive site Ser563, accompanied with glycerol release at the 1-week time-point, contributing to the partial normalization of adipocyte volume with respect to control (N) animals. After two weeks, when the adipocyte volumes of HFD-3,5-T2 rats were completely normalized to those of the controls (N), 3,5-T2 consistently induced HSL phosphorylation at Ser563, indicative of a combined effect of 3,5-T2-induced adipose lipolysis and increasing non-adipose oxidative metabolism. VAT proteome analysis after 4 weeks of treatment revealed that 3,5-T2 significantly altered the proteomic profile of HFD rats and produced a marked pro-angiogenic action. This was associated with a reduced representation of proteins involved in lipid storage or related to response to oxidative stress, and a normalization of the levels of those involved in lipogenesis-associated mitochondrial function. In conclusion, the prevention of VAT mass-gain by 3,5-T2 occurred through different molecular pathways that, together with the previously reported stimulation of resting metabolism and liver fatty acid oxidation, are associated with an anti adipogenic/lipogenic potential and positively impact on tissue health.",
keywords = "3,5-diiodo-L-thyronine, ATGL, hormone sensitive lipase, lipolysis, proteomics, visceral white adipose tissue",
author = "Elena Silvestri and Rosalba Senese and Federica Cioffi and {De Matteis}, Rita and Davide Lattanzi and Assunta Lombardi and Antonia Giacco and Salzano, {Anna Maria} and Andrea Scaloni and Michele Ceccarelli and Maria Moreno and Fernando Goglia and Antonia Lanni and {de Lange}, Pieter",
year = "2019",
month = "1",
day = "27",
doi = "10.3390/nu11020278",
language = "English",
volume = "11",
journal = "Nutrients",
issn = "2072-6643",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

TY - JOUR

T1 - 3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet

AU - Silvestri, Elena

AU - Senese, Rosalba

AU - Cioffi, Federica

AU - De Matteis, Rita

AU - Lattanzi, Davide

AU - Lombardi, Assunta

AU - Giacco, Antonia

AU - Salzano, Anna Maria

AU - Scaloni, Andrea

AU - Ceccarelli, Michele

AU - Moreno, Maria

AU - Goglia, Fernando

AU - Lanni, Antonia

AU - de Lange, Pieter

PY - 2019/1/27

Y1 - 2019/1/27

N2 - When administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 μg/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white adipose tissue (VAT) contributed to the beneficial effect of 3,5-T2 over time (between 1 day and 4 weeks following administration). 3,5-T2 programmed the adipocyte for lipolysis by rapidly inducing hormone sensitive lipase (HSL) phosphorylation at the protein kinase A-responsive site Ser563, accompanied with glycerol release at the 1-week time-point, contributing to the partial normalization of adipocyte volume with respect to control (N) animals. After two weeks, when the adipocyte volumes of HFD-3,5-T2 rats were completely normalized to those of the controls (N), 3,5-T2 consistently induced HSL phosphorylation at Ser563, indicative of a combined effect of 3,5-T2-induced adipose lipolysis and increasing non-adipose oxidative metabolism. VAT proteome analysis after 4 weeks of treatment revealed that 3,5-T2 significantly altered the proteomic profile of HFD rats and produced a marked pro-angiogenic action. This was associated with a reduced representation of proteins involved in lipid storage or related to response to oxidative stress, and a normalization of the levels of those involved in lipogenesis-associated mitochondrial function. In conclusion, the prevention of VAT mass-gain by 3,5-T2 occurred through different molecular pathways that, together with the previously reported stimulation of resting metabolism and liver fatty acid oxidation, are associated with an anti adipogenic/lipogenic potential and positively impact on tissue health.

AB - When administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 μg/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white adipose tissue (VAT) contributed to the beneficial effect of 3,5-T2 over time (between 1 day and 4 weeks following administration). 3,5-T2 programmed the adipocyte for lipolysis by rapidly inducing hormone sensitive lipase (HSL) phosphorylation at the protein kinase A-responsive site Ser563, accompanied with glycerol release at the 1-week time-point, contributing to the partial normalization of adipocyte volume with respect to control (N) animals. After two weeks, when the adipocyte volumes of HFD-3,5-T2 rats were completely normalized to those of the controls (N), 3,5-T2 consistently induced HSL phosphorylation at Ser563, indicative of a combined effect of 3,5-T2-induced adipose lipolysis and increasing non-adipose oxidative metabolism. VAT proteome analysis after 4 weeks of treatment revealed that 3,5-T2 significantly altered the proteomic profile of HFD rats and produced a marked pro-angiogenic action. This was associated with a reduced representation of proteins involved in lipid storage or related to response to oxidative stress, and a normalization of the levels of those involved in lipogenesis-associated mitochondrial function. In conclusion, the prevention of VAT mass-gain by 3,5-T2 occurred through different molecular pathways that, together with the previously reported stimulation of resting metabolism and liver fatty acid oxidation, are associated with an anti adipogenic/lipogenic potential and positively impact on tissue health.

KW - 3,5-diiodo-L-thyronine

KW - ATGL

KW - hormone sensitive lipase

KW - lipolysis

KW - proteomics

KW - visceral white adipose tissue

UR - http://www.scopus.com/inward/record.url?scp=85060592896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060592896&partnerID=8YFLogxK

U2 - 10.3390/nu11020278

DO - 10.3390/nu11020278

M3 - Article

VL - 11

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 2

ER -